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A novel candidate effector CcAA9-20333 from Colletotrichum coccodes: identification, expression, and functional site
Ting Ma1, Chengde Yang1, Mengjun Jin1
1College of Plant Protection, Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Gansu Agricultural University, Lanzhou, 730070, China.
Microbial Pathogenesis
|June 24, 2025
Summary
This study identifies and characterizes a novel lytic polysaccharide monooxygenase (LPMO) gene, CcAA9-20333, from Colletotrichum coccodes, crucial for potato anthracnose pathogenicity. The characterized enzyme shows high stability and activity, with mutations affecting its virulence.
Area of Science:
- Biochemistry
- Molecular Biology
- Plant Pathology
Background:
- Auxiliary activity family 9 (AA9) lytic polysaccharide monooxygenases (LPMOs) are copper enzymes that degrade polysaccharides.
- The specific LPMO gene responsible for pathogenicity in Colletotrichum coccodes, a potato anthracnose pathogen, requires further investigation.
Purpose of the Study:
- To identify and characterize the AA9 LPMO gene CcAA9-20333 from Colletotrichum coccodes.
- To investigate the role of CcAA9-20333 in the pathogenicity of potato anthracnose.
Main Methods:
- Mining transcriptome data to identify the candidate gene CcAA9-20333.
- Cloning, prokaryotic expression, purification, and enzymatic characterization of CcAA9-20333.
- Site-directed mutagenesis to create mutants and assess pathogenicity and substrate binding sites.
Main Results:
- CcAA9-20333 exhibits optimal activity at 40-60°C and pH 6.0, with high thermostability and pH stability.
- The enzyme releases reducing sugars and displays substrate specificity.
- Mutations in CcAA9-20333 reduced pathogenicity in Solanaceae plants compared to the wild-type.
Conclusions:
- The LPMO gene CcAA9-20333 from C. coccodes is characterized and shown to play a role in pathogenicity.
- This study provides the first report on CcAA9-20333 and its contribution to potato anthracnose.
- Understanding CcAA9-20333 function can inform strategies for managing potato anthracnose.
