Laccase Activity of Trametes versicolor Using Various Pineapple and Arabica Coffee Wastes Under Solid-State
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<b>Background and Objective:</b> Laccase, a ligninolytic enzyme, can degrade lignin by utilizing lignocellulose. With this ability, laccase is useful as a pre-treatment enzyme of lignocellulosic materials in various industries. This study evaluated the laccase activity produced by <i>T. versicolor</i> using pineapple and coffee-derived waste as lignocellulosic-rich substrates using solid fermentation. <b>Materials and Methods:</b> This study tested laccase production activities from various pineapple wastes and coffee peels through a solid fermentation process by growing <i>T. versicolor</i> on the substrate mixture of Szabo media and pineapple skin or coffee peel. The laccase produced was analyzed for its activity and growth, qualitatively and quantitatively during a 12 days incubation time. The method used was experimental followed by descriptive analysis. <b>Results:</b> Various amounts of lignocellulose were obtained among pineapple wastes and coffee peel. Laccase from these substrates was optimally produced on day 6 incubation on Szabo+5 g coffee peel group showed the highest laccase activity levels, following its biomass weight (1949.13 U/L and 3.498 g). The optimum pH for laccase production was reached in the range 4-6. The produced laccase was indicated by the appearance of the blackish-brown zone on PDA agar supplemented with guaiacol. <b>Conclusion:</b> Lignocellulosic wastes from pineapple and coffee had the potential as substrates to produce laccase from <i>T. versicolor</i> with various activity levels which day 6 incubation showed the most optimum fermentation period.
