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Related Experiment Video

Updated: Sep 18, 2025

Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation
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Ribosome Incorporation Transdifferentiates Chick Primary Cells and Induces Their Proliferation by Secreting Growth

Shota Inoue1, Arif Istiaq1, Anamika Datta1

  • 1Department of Stem Cell Biology, Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan.

Journal of Developmental Biology
|June 25, 2025
PubMed
Summary

Ribosome incorporation into non-mammalian cells triggers transdifferentiation, creating multipotent cells. This breakthrough, combined with the CulNet System, offers a scalable path for sustainable cultured meat production.

Keywords:
cell proliferationchick primary cellscultured meatribosomes

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Area of Science:

  • Cell Biology
  • Biotechnology
  • Developmental Biology

Background:

  • Mammalian cells (human dermal fibroblasts) transdifferentiate via lactic acid bacteria (LAB)-derived ribosomes, forming ribosome-induced cell clusters (RICs).
  • RICs exhibit multi-germ layer differentiation potential, suggesting a universal mechanism.

Purpose of the Study:

  • To investigate ribosome incorporation in non-mammalian cells for transdifferentiation.
  • To explore commercial applications in cell-cultured meat production.

Main Methods:

  • Ribosome incorporation into chick primary muscle-derived cells (CMCs).
  • Analysis of CMC transdifferentiation into adipocytes, osteoblasts, and chondrocytes.
  • RNA-sequencing (RNA-seq) to assess gene expression.
  • Development of the "CulNet System" for cell-cultured meat.

Main Results:

  • Ribosome incorporation successfully transdifferentiated CMCs into multiple cell lineages.
  • Supernatant from ribosome-incorporated CMCs enhanced proliferation.
  • RICs-CMC showed increased expression of multi-lineage growth genes.
  • The CulNet System was developed to mimic organ interactions.

Conclusions:

  • Ribosome incorporation is a universal mechanism for cell transdifferentiation across species.
  • This approach, coupled with the CulNet System, enhances scalability and cost-effectiveness for cultured chicken meat.
  • Offers a sustainable alternative to traditional meat production.