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Nanofibrous Membranes Based on Collagen and Conductive Polymers with Perspective for Biological Applications
Tonantzi Pérez-Moreno1, Claudia D'Urso2, Gabriel Trejo3
1Facultad de Ingenieía, División de Investigación y Posgrado, Universidad Autónoma de Querérato, Santiago de Querétaro 76010, Querétaro, Mexico.
Membranes
|June 25, 2025
Summary
Collagen-chitosan membranes blended with conductive polymers like polyaniline and polypyrrole show promise for tissue regeneration. These biocompatible scaffolds exhibit varying mechanical properties and ion diffusion resistance, suitable for specific regenerative applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Polymer Chemistry
Background:
- Collagen-chitosan (C-Ch) composites are explored for biomedical applications.
- Conductive polymers (CPs) like polyaniline (Pani) and polypyrrole (Ppy) offer unique electrical properties.
- Developing advanced scaffolds requires understanding material interactions and performance.
Purpose of the Study:
- To fabricate and characterize collagen-chitosan membranes incorporating conductive polymers (polyaniline and polypyrrole).
- To evaluate the morphological, mechanical, ion diffusion, and biocompatibility properties of these novel composite membranes.
- To assess the potential of these C-Ch/CP composites for electrically active scaffolds and tissue-specific regeneration.
Main Methods:
- Electrospinning of collagen-chitosan with polyaniline and polypyrrole using non-toxic solvents (PBS, ethanol).
- Scanning Electron Microscopy (SEM) for morphological analysis after swelling.
- Fourier-Transform Infrared (FTIR) spectroscopy to confirm C-Ch and CP interactions.
- Mechanical testing (tensile/stress) and ion diffusion studies (Na+, Ca2+).
- Preliminary in vitro biocompatibility testing using H9C2 cells.
Main Results:
- SEM revealed morphological changes post-swelling.
- FTIR confirmed specific interactions between collagen-chitosan and conductive polymers.
- C-Ch/Ppy membranes demonstrated superior elasticity and stress distribution compared to C-Ch/Pani.
- Both membrane types exhibited higher resistance to Ca2+ ion diffusion than Na+.
- H9C2 cells showed successful adhesion to the fabricated membranes.
Conclusions:
- Collagen-chitosan/polyaniline composites are suitable for electrically active scaffolds.
- Collagen-chitosan/polypyrrole composites show potential for mechanically dynamic tissue regeneration.
- These C-Ch/CP composite membranes represent promising biomaterials for advanced regenerative medicine applications.
