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Related Concept Videos

Dialysis01:15

Dialysis

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Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...
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  11. Selective Removal Of Plasma Proteins By Double-filtration Plasmapheresis In Canine Blood: An Ex Vivo Study And Retrospective Report Of In Vivo Clinical Treatments In Three Dogs.
  1. Home
  3. Research Domains
  5. Agricultural, Veterinary And Food Sciences
  7. Veterinary Sciences
  9. Veterinary Medicine (excl. Urology)
  11. Selective Removal Of Plasma Proteins By Double-filtration Plasmapheresis In Canine Blood: An Ex Vivo Study And Retrospective Report Of In Vivo Clinical Treatments In Three Dogs.

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Selective Removal of Plasma Proteins by Double-Filtration Plasmapheresis in Canine Blood: An Ex Vivo Study and Retrospective Report of In Vivo Clinical Treatments in Three Dogs.

Roberta Troia1, Claudia Iannucci1, Lisa Niemann1

  • 1Section of Small Animal Emergency and Critical Care, Department of Small Animals, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland.

Veterinary Sciences
|June 25, 2025

View abstract on PubMed

Summary
This summary is machine-generated.
Keywords:
INUSpheresis®dogdouble-filtration plasmapheresisextracorporeal apheresis

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Double-filtration plasmapheresis (DFPP) selectively removes plasma proteins in dogs. While effective at 1.5 plasma volumes (PV), selectivity decreases with higher PVs, impacting albumin and fibrinogen levels.

Area of Science:

  • Veterinary Medicine
  • Nephrology
  • Plasma Exchange

Background:

  • Double-filtration plasmapheresis (DFPP) is a therapeutic apheresis technique for selective removal of high-molecular-weight proteins.
  • Limited data exists on DFPP efficacy and protein loss in canine models.
  • Understanding protein fraction loss is crucial for optimizing DFPP protocols in veterinary medicine.

Purpose of the Study:

  • To quantitatively assess the net loss of specific plasma protein fractions during ex vivo canine blood processing via DFPP.
  • To evaluate the impact of processing different plasma volumes (PVs) on protein selectivity.
  • To describe in vivo DFPP application and protein removal in dogs.

Main Methods:

  • Ex vivo canine blood was processed using INUSpheresis® DFPP at 1.5, 2, and 3 plasma volumes (PVs).
immunoglobulin
  • Plasma protein fractions (albumin, globulin, fibrinogen) were measured pre- and post-treatment.
  • Five DFPP treatments were performed in three dogs to assess in vivo protein removal.

    • Main Results:

    • At 1.5 PV, net globulin and albumin loss was 41% and 25%, respectively.
    • At 3 PV, net globulin and albumin loss increased to 47% and 40%, respectively.
    • Fibrinogen was undetectable after 1.5 PV processing; in vivo results showed significantly lower albumin reduction compared to globulin and fibrinogen.

    Conclusions:

    • INUSpheresis® demonstrates semi-selective protein removal in dogs, with better albumin preservation at 1.5 PV.
    • Protein removal selectivity diminishes with increasing processed plasma volumes.
    • DFPP shows potential for therapeutic apheresis in dogs, but protocol optimization is needed.