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Effect of laser applications with different agents on dentin surface roughness, bacterial adhesion and tubule
Ayşe Dündar1, Kaan Yılmaz2, Çağatay Barutçugil3
1Department of Restorative Dentistry, Faculty of Dentistry, Akdeniz University, Antalya, 07058, Turkey. ayse_dent@hotmail.com.
Objective:
This study aimed to evaluate the effects of laser application with different agents on dentin surface roughness, tubule occlusion, and bacterial adhesion in vitro.
Materials And Methods:
A total of 216 dentin blocks from bovine incisors were divided into nine groups. Treatments included 5% sodium fluoride (NaF) varnish, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), Er, Cr: YSGG laser, and diode laser in various combinations. Surface roughness (SR) was measured using a profilometer. S. mutans and S. mitis suspensions were incubated for 24 h, and bacterial adhesion was quantified (×10⁸ Cfu/ml) and visualized using confocal laser scanning microscopy. Tubule occlusion was assessed via SEM imaging and analyzed with ImageJ software. Statistical analysis was performed using one-way ANOVA and Tukey HSD (p = 0.05).
Results:
Except for the CPP-ACP groups treated with laser, all groups exhibited significantly higher surface roughness (p < 0.05); however, this did not influence bacterial adhesion (p > 0.05). All treatments significantly reduced the number and diameter of open dentinal tubules (p < 0.05). The combination of NaF with Er, Cr: YSGG and diode laser enhanced tubule occlusion, while CPP-ACP with laser had no additional effect.
Conclusions:
Increased surface roughness, except in CPP-ACP with laser groups, did not affect bacterial adhesion. All desensitization methods effectively promoted tubule occlusion, with the diode laser demonstrating greater efficacy than the Er, Cr: YSGG laser.
Clinical Significance:
Lasers should be used with caution as they may increase the roughness of the dentin surface. The combined application of NaF with lasers may improve its clinical efficacy.
