Related Experiment Video
Updated: Sep 18, 2025

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
The ERF5-WOX4 transcription factor regulatory module controls mannan biosynthesis in the orchid Dendrobium officinale
Danqi Zeng1,2, Hongyu Shi1,3, Jing Chen1,3
1Guangdong Provincial Key Laboratory of Applied Botany, State Key Laboratory of Plant Diversity and Specialty Crops, Key Laboratory of National Forestry and Grassland Administration on Plant Conservation and Utilization in Southern China, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Abstract:
Plants produce mannans, which compose a class of polysaccharides with considerable potential for health-related applications. GDP-mannose pyrophosphorylase (GMP) plays a pivotal role in mannan biosynthesis by facilitating the formation of GDP-mannose. In this study, we identified the WUSCHEL (WUS)-related homeobox (WOX) transcription factor DoWOX4 as a candidate protein that interacts with the DoGMP1 promoter in Dendrobium officinale through yeast one-hybrid library screening. We demonstrated that DoWOX4 directly binds to the DoGMP1 promoter and activates its expression. DoWOX4 overexpression significantly enhanced mannan biosynthesis, whereas silencing DoWOX4 inhibited this process. Furthermore, we found that the ethylene response factor 5 (DoERF5) binds to the DoWOX4 promoter in vitro. Subsequent analyses revealed that DoERF5 negatively regulates DoWOX4 and mannan biosynthesis. This study deepens our understanding of the regulatory mechanisms governing mannan biosynthesis in plants and provides valuable genetic resources for plant bio-breeding initiatives.
Related Concept Videos
Master Transcription Regulators
Canonical Wnt Signaling Pathway
Gene Regulation During Sporulation
Transcriptional Regulation: Riboswitches
Riboswitches
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon...

