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Dual-mode colorimetric-SPR immunosensor based on gold nanoparticles for sCD146 detection in plasma.

Zhaochen Huo1, Qilong Li2, Miaomiao Liu2

  • 1School of Electrical and Information Engineering, Zhengzhou University, Henan, Zhengzhou, 450001, China.

Biosensors & Bioelectronics
|June 25, 2025
PubMed
Summary

A novel dual-mode immunosensor detects soluble CD146 (sCD146), a cancer biomarker, in plasma. This advancement offers a sensitive and specific method for early cancer detection and prognosis.

Keywords:
AuNPsColorimetric detectionDual-mode biosensorSurface plasmon resonancesCD146

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Cancer Diagnostics

Background:

  • Soluble cluster of differentiation 146 (sCD146) is implicated in tumor metastasis, cell signaling, migration, proliferation, and inflammation.
  • Plasma sCD146 detection is a promising strategy for early cancer screening.

Purpose of the Study:

  • To develop a rapid, highly specific dual-mode immunosensor for sCD146 detection.
  • To integrate colorimetry and surface plasmon resonance (SPR) for enhanced sensitivity and specificity.

Main Methods:

  • Synthesis of bovine serum albumin (BSA)-modified gold nanoparticles (AuNPs) with peroxidase (POD) activity and SPR properties.
  • Development of a dual-mode immunosensor combining colorimetric and SPR detection techniques.
  • Optimization of assay conditions for sCD146 detection in plasma.

Main Results:

  • The immunosensor demonstrated a linear detection range of 5-600 ng/mL for sCD146.
  • Achieved low limits of detection (LODs) of 4 ng/mL (colorimetric) and 1 ng/mL (SPR).
  • Results showed excellent agreement with conventional ELISA (P > 0.05).

Conclusions:

  • The developed AuNP-based dual-mode immunosensor is a versatile and efficient platform for blood biomarker analysis.
  • This technology holds significant potential for early cancer diagnosis, prognosis prediction, and clinical applications.
  • Offers a novel approach for advancing cancer diagnostics and research.