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Published on: February 19, 2016
Flexible ag-SiO2 microsphere SERS substrate integrated microfluidic Chip for fungal pathogen detection in rapeseed
Wang Peng1, Chao Yi2, Yuankai Zhang2
1College of Engineering, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Agricultural Equipment in Mid-Lower Yangtze River, Ministry of Agriculture and Rural Affairs, Wuhan 430070, China; Key Laboratory of Smart Farming Technology for Agricultural Animals, Ministry of Agriculture and Rural Affairs, Wuhan 430070, China; Engineering Research Center of Intelligent Technology for Agriculture, Ministry of Education, Wuhan 430070, China.
None:
Rapeseed Sclerotinia stem rot, caused by Sclerotinia fungi, is a devastating agricultural disease-causing massive yield losses globally. However, the current rapeseed Sclerotinia stem rot detection and control using Raman spectroscopy is limited, and unable to meet the demand for high-precision detection and integration of various types of Sclerotinia stem rot fungus. In this study, a flexible 3D core-shell Ag-SiO2 microsphere SERS substrate integrated with a pumpless multichannel microfluidic chip was proposed. The flexible SERS substrates were used for precise identification fungus such as Sclerotinia sclerotiorum and Sclerotinia minor. The flexible Ag-SiO2 microsphere SERS substrate demonstrated a linear detection range of 10-2-10-12 M for Rhodamine 6G (R2 = 0.958), achieving an enhancement factor of 8.5 × 1010 at 1506 cm-1 with 6.93 % RSD and 10-12 M detection limit. The proposed flexible SERS microfluidic chip provides a promising application for microbial detection and analysis.
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