Ratiometric electrochemiluminescence biosensor based on Ru(dcbpy)₃²⁺ and TiO₂ quantum dots for the detection of...

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Biochemistry

Background:

• Urokinase (UK) is a key biomarker for renal function, cardiovascular diseases, and inflammation.
• Accurate and sensitive detection of UK is crucial for clinical diagnosis.

Purpose of the Study:

• To construct a novel sandwich-type ratiometric electrochemiluminescence (ECL) biosensor for urokinase (UK) detection.
• To evaluate the biosensor's sensitivity, accuracy, and applicability in real-world samples.

Main Methods:

• Fabrication of a ratiometric ECL biosensor using titanium dioxide magnetic beads (P25) and a Tb-GMP ICPn complex with Ru(dcbpy)22+.
• Optimization of experimental conditions using potassium persulfate and tri-n-propylamine as co-reactants.
• Detection of UK via measuring changes in ECL intensities.

Main Results:

• A linear correlation was observed between ECL intensities and UK concentration (10^-2 to 10^4 ng/mL).
• Achieved low limits of detection (LODs) of 7.2 pg/mL and 5.8 pg/mL.
• Demonstrated satisfactory performance in detecting UK in real-world samples.

Conclusions:

• The developed ratiometric ECL biosensor offers a sensitive and accurate method for UK detection.
• This biosensor shows significant potential for clinical detection and diagnosis of diseases related to UK levels.