Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Precision Intrinsic Interactome Elucidation of Chimeric Antigen Receptors via Photocatalytic Micromapping (μMap-CAR).

Journal of the American Chemical Society·2026
Same author

From randomness to recognition: modeling the evolution of DNA sequence information during enrichment for binding.

Bioinformatics advances·2026
Same author

Integrating mortality surveillance as a routine component of national essential public health functions: a blueprint for action.

BMJ public health·2026
Same author

Electrical stimulation induces differentiation onset consistent with a therapeutic approach in neuroblastoma cells.

Scientific reports·2026
Same author

Real-World Effectiveness of Risankizumab in Refractory Crohn's Disease: The RISANCROHN Study From the ENEIDA Registry.

Alimentary pharmacology & therapeutics·2026
Same author

Piecewise Stereoselective Assembly of Multisubstituted Alkenes.

Journal of the American Chemical Society·2026
Same journal

Proton-Gated Torsional Spring for Molecular Energy Storage.

Journal of the American Chemical Society·2026
Same journal

Topologically Programmed Dual-Channel Covalent Organic Frameworks Decouple Gas and Ion Fluxes for Acidic CO<sub>2</sub> Electroreduction.

Journal of the American Chemical Society·2026
Same journal

Plasmonic Re-Excitation Enables Superoxide-Mediated Ethane Conversion to Acetic Acid under Visible Light.

Journal of the American Chemical Society·2026
Same journal

Photocatalytic Controlled Halodefluorination of Perfluoroalkyl Compounds Using <i>N</i>-Arylphenothiazines.

Journal of the American Chemical Society·2026
Same journal

Photoinduced Disproportionation Enables Oxidative Addition of Aryl Iodides at a Gallium(I) Center.

Journal of the American Chemical Society·2026
Same journal

Biocatalytic C3 β-<i>O</i>-Glycosylation of Triterpenes and Sterols to Synthesize Natural and Unnatural Saponins.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: Sep 18, 2025

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
09:20

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Published on: September 2, 2013

27.8K

μMap-FFPE: A High-Resolution Protein Proximity Labeling Platform for Formalin-Fixed Paraffin-Embedded Tissue Samples.

Noah B Bissonnette1, Marie E Zamanis2, Steve D Knutson1

  • 1Merck Center for Catalysis at Princeton University, Princeton, New Jersey 08544, United States.

Journal of the American Chemical Society
|June 26, 2025
PubMed
Summary
This summary is machine-generated.

We developed μMap-FFPE, a novel method to map protein interactions in formalin-fixed paraffin-embedded tissues. This technique allows studying cellular microenvironments in preserved patient samples, advancing disease research.

More Related Videos

Author Spotlight: FISH as a Tool for Precise Gene Amplification Assessment in Cancer Specimens
03:55

Author Spotlight: FISH as a Tool for Precise Gene Amplification Assessment in Cancer Specimens

Published on: July 12, 2024

1.5K
Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
08:18

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

1.8K

Related Experiment Videos

Last Updated: Sep 18, 2025

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
09:20

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Published on: September 2, 2013

27.8K
Author Spotlight: FISH as a Tool for Precise Gene Amplification Assessment in Cancer Specimens
03:55

Author Spotlight: FISH as a Tool for Precise Gene Amplification Assessment in Cancer Specimens

Published on: July 12, 2024

1.5K
Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
08:18

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

1.8K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Understanding disease states requires elucidating small-scale biomolecular protein interaction networks (microenvironments).
  • Photoproximity labeling, such as μMap, offers high-resolution mapping of spatial relationships within subcellular architectures.
  • Existing in vitro models lack the cell-type heterogeneity and 3D structure crucial for clinical relevance.

Purpose of the Study:

  • To develop a photoproximity labeling method compatible with formalin-fixed paraffin-embedded (FFPE) tissues.
  • To enable the study of protein interactions within the native cellular environment of FFPE samples.
  • To compare the CD20 interactome across healthy, cancerous, and patient-derived tissues.

Main Methods:

  • Introduction of μMap-FFPE, a novel labeling system.
  • Adaptation of photoproximity labeling techniques for FFPE tissues, overcoming chemical modification incompatibilities.
  • Application of μMap-FFPE to analyze the CD20 interactome.

Main Results:

  • Successfully adapted photoproximity labeling for FFPE tissues.
  • Enabled comparison of the CD20 interactome in diverse cellular contexts.
  • Demonstrated the utility of μMap-FFPE for studying protein interactions in preserved patient samples.

Conclusions:

  • μMap-FFPE is a valuable tool for mapping protein interactions in FFPE tissues.
  • This method facilitates the study of cellular microenvironments in native tissue architectures.
  • Findings from μMap-FFPE can provide critical clinical insights into disease states.