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Updated: Sep 18, 2025
10:06
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
Published on: April 26, 2017
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Alternative Splicing in Tumorigenesis and Cancer Therapy
Huiping Chen1,2,3, Jingqun Tang1,4, Juanjuan Xiang1,2,3
1Hunan Key Laboratory of Early Diagnosis and Precise Treatment of Lung Cancer, The Second Xiangya Hospital, Central South University, Changsha 410013, China.
Biomolecules
|June 26, 2025
Summary
Alternative splicing (AS) dysregulation drives cancer by creating oncogenic proteins and neoantigens. Targeting AS with splice-switching oligonucleotides offers a promising avenue for personalized cancer therapies.
Area of Science:
- Molecular Biology
- Genetics
- Cancer Research
Background:
- Alternative splicing (AS) generates proteome diversity from single genes.
- Splicing factors (SFs) like SR proteins and hnRNPs regulate AS.
- AS dysregulation is linked to cancer development and progression.
Purpose of the Study:
- To review AS dysregulation mechanisms in cancer.
- To explore AS's role in tumor progression and therapeutic targeting.
- To discuss novel technologies and future prospects for AS-targeted cancer therapies.
Main Methods:
- Review of existing literature on AS in cancer.
- Discussion of splicing factors and their regulatory roles.
- Exploration of high-throughput sequencing and computational approaches.
Main Results:
- Aberrant AS can produce oncogenic isoforms promoting tumorigenesis and therapy resistance.
- AS abnormalities can lead to loss of tumor suppressor activity.
- Abnormal AS generates neoantigens, potential targets for cancer immunotherapy.
Conclusions:
- Targeting AS offers potential for personalized cancer therapies.
- Splice-switching oligonucleotides (SSOs) show therapeutic promise.
- Innovative technologies are advancing AS research in oncology.
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