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Related Concept Videos

Cell Culture01:21

Cell Culture

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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
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Correction: Komatsu et al. Three-Dimensional Visualization and Detection of the Pulmonary Venous-Left Atrium Connection Using Artificial Intelligence in Fetal Cardiac Ultrasound Screening. <i>Bioengineering</i> 2026, <i>13</i>, 100.

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Updated: Sep 18, 2025

An Innovative 3D-Printed Insert Designed to Enable Straightforward 2D and 3D Cell Cultures
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Advanced 3D Cell Culture Technologies and Formats.

Cornelia Kasper1, Dominik Egger2

  • 1Department of Biotechnology and Food Science, BOKU University, 1190 Vienna, Austria.

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|June 26, 2025
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Summary
This summary is machine-generated.

This special issue explores advanced 3D cell culture technologies. Innovations enhance the physiological relevance and therapeutic applications of cells and cell-derived products.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Tissue Engineering

Background:

  • Three-dimensional (3D) cell culture models are increasingly vital for biological research.
  • Traditional 2D cultures often fail to replicate the complex in vivo microenvironment.
  • Innovations in 3D cell culture aim to bridge this gap, improving experimental outcomes.

Discussion:

  • This special issue highlights novel 3D cell culture technologies and formats.
  • Articles cover advancements in biomaterials, scaffold design, and bioreactor systems.
  • The focus is on increasing the physiological relevance of in vitro models.

Key Insights:

  • 3D cell culture systems offer superior recapitulation of in vivo cellular behavior and tissue architecture.
  • These advanced models enhance the predictive power of drug screening and disease modeling.
  • The therapeutic utility of cells and cell-derived products is significantly improved.

Outlook:

  • Future research will likely focus on integrating multi-omics data with 3D culture systems.
  • The development of organ-on-a-chip technologies and complex tissue models is a key trend.
  • Wider adoption of advanced 3D cultures promises to accelerate regenerative medicine and personalized therapies.