Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

5.2K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.2K
Classification of Skeletal Muscle Fibers01:48

Classification of Skeletal Muscle Fibers

56.9K
Skeletal muscles continuously produce ATP to provide the energy that enables muscle contractions. Skeletal muscle fibers can be categorized into three types based on differences in their contraction speed and how they produce ATP, as well as physical differences related to these factors. Most human muscles contain all three muscle fiber types, albeit in varying proportions.
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
56.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recurrent hypersomnia following infections: A Kleine-Levin syndrome-like presentation with thalamic hypoperfusion.

Psychiatry and clinical neurosciences·2026
Same author

Analysis of intracellular organelles in neurons differentiated from iPSCs of Chédiak-Higashi syndrome patients.

Pediatrics international : official journal of the Japan Pediatric Society·2026
Same author

TAF4B knockdown differentially affects erythroid and natural killer cells but not monocytic differentiation from human cord blood HSPCs.

Biochemical and biophysical research communications·2025
Same author

Photobiomodulation as a therapeutic approach for attention-deficit/hyperactivity disorder in model rats.

Lasers in medical science·2025
Same author

A pluripotent stem cell model of Emberger syndrome reveals reduced lymphatic endothelial differentiation.

International journal of hematology·2025
Same author

Improvement of multilineage hematopoiesis in hematopoietic stem cell-transferred c-kit mutant NOG-EXL humanized mice.

Stem cell research & therapy·2024

Related Experiment Video

Updated: Sep 18, 2025

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues
09:30

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

Published on: February 18, 2021

4.3K

Enhancing Skeletal Muscle Fiber Type Transition Through Substrate Coating Alteration in Myoblast Cell Culture.

Yhusi Karina Riskawati1,2, Chuang-Yu Lin3, Akira Niwa4

  • 1International Ph.D. Program in Cell Therapy and Regenerative Medicine, College of Medicine, Taipei Medical University, Taipei 110301, Taiwan.

International Journal of Molecular Sciences
|June 26, 2025
PubMed
Summary

Extracellular matrix components like Collagen I promote slow muscle fiber development, while fibronectin and Geltrex™ promote fast muscle fiber development, offering insights into muscle regeneration therapies.

Keywords:
Collagen IFibronectinGeltrex™, C2C12 differentiationmuscle fiber typetranscriptomic

More Related Videos

In Vitro Differentiation of Mature Myofibers for Live Imaging
08:12

In Vitro Differentiation of Mature Myofibers for Live Imaging

Published on: January 7, 2017

10.4K
Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation
08:38

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation

Published on: March 19, 2013

21.2K

Related Experiment Videos

Last Updated: Sep 18, 2025

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues
09:30

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

Published on: February 18, 2021

4.3K
In Vitro Differentiation of Mature Myofibers for Live Imaging
08:12

In Vitro Differentiation of Mature Myofibers for Live Imaging

Published on: January 7, 2017

10.4K
Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation
08:38

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation

Published on: March 19, 2013

21.2K

Area of Science:

  • Muscle biology and regenerative medicine.

Background:

  • Skeletal muscle diseases present clinical challenges, and the extracellular matrix (ECM) influences muscle regeneration.
  • The precise mechanisms by which ECM components regulate muscle fiber type specification are not fully understood.

Purpose of the Study:

  • To investigate how different extracellular matrix (ECM) substrates influence skeletal muscle fiber type differentiation.
  • To elucidate the molecular pathways mediating ECM-driven muscle fiber type specification.

Main Methods:

  • C2C12 myoblasts were differentiated on fibronectin, Collagen I, and Geltrex™-coated plates.
  • Quantitative polymerase chain reaction (qPCR), Western blot, immunofluorescence, and RNA sequencing were employed.
  • Analysis focused on myogenic marker expression and fiber type-specific gene and protein profiles.

Main Results:

  • Collagen I significantly promoted slow-type muscle fiber development, indicated by increased slow fiber markers and associated gene expression.
  • Fibronectin and Geltrex™ promoted fast-type muscle fiber development, with elevated fast fiber markers and gene expression.
  • Specific signaling pathways, including calcineurin/NFAT, MEF2, AMPK, PI3K/AKT, ERK1, HIF1A, FOXO1, and NFKB, were implicated in mediating these ECM effects.

Conclusions:

  • Extracellular matrix components differentially regulate skeletal muscle fiber type specification.
  • These findings provide mechanistic insights into ECM-mediated muscle regeneration and offer potential targets for therapeutic interventions in muscle diseases.