Related Experiment Video
Updated: Sep 18, 2025

11:46
Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
Published on: May 19, 2018
12.6K
3D Printability of Lysine-Modified Myofibrillar Protein Emulsions
Lin Liao1, Zilan Feng2, Yoon-Yen Yow3
1School of Food Science and Engineering, Hainan Tropical Ocean University, Sanya 572022, China.
Foods (Basel, Switzerland)
|June 26, 2025
Summary
Lysine and tilapia myofibrillar protein composite particles create stable emulsions for 3D printing. This lysine-enhanced protein formulation improves emulsion properties, enabling precise and clear 3D printed food structures.
Area of Science:
- Food Science
- Materials Science
- Biotechnology
Background:
- Highly inwardly directed emulsions (HIPEs) are crucial in food formulation.
- Tilapia myofibrillar protein (MP) has potential as an emulsifier.
- Enhancing MP properties is key for advanced applications like 3D printing.
Purpose of the Study:
- To investigate the interaction between lysine (Lys) and tilapia myofibrillar protein (MP).
- To formulate and characterize Lys-MP composite particles for HIPE stabilization.
- To evaluate the suitability of Lys-MP stabilized HIPEs for 3D printing applications.
Main Methods:
- Spectroscopic analysis (Infrared spectroscopy)
- Potentiometric analysis
- Molecular docking studies
- Rheological analysis
- Particle size and structural analysis
Main Results:
- Lysine interacts with MP via hydrogen bonding and electrostatic forces, altering MP structure and solubility.
- Lys-MP composite particles significantly enhance HIPE stability and viscoelasticity compared to MP alone.
- Lys-MP stabilized HIPEs exhibit improved energy storage modulus (G' up to 1002.10 Pa) and reduced droplet size (10.84 μm).
- HIPEs stabilized with 1.5 wt% Lys-MP demonstrate superior print accuracy (91.36%), resolution, and clarity for 3D printing.
Conclusions:
- Lys-MP composite particles offer a novel approach to stabilize HIPEs for advanced food applications.
- The enhanced rheological properties of Lys-MP stabilized HIPEs are ideal for 3D printing inks.
- This study presents a promising strategy for developing tailored composite particles for 3D printing food technologies.

