Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in polyamine synthesis and concentrations during chick embryo development.

B Löwkvist, H Emanuelsson, O Heby

    The Journal of Experimental Zoology
    |June 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Data of the Insect Biome Atlas: a metabarcoding survey of the terrestrial arthropods of Sweden and Madagascar.

    Scientific data·2025
    Same author

    Pharmacodynamics, pharmacokinetics and safety of ticagrelor in Asian patients with stable coronary artery disease.

    Cardiovascular intervention and therapeutics·2014
    Same author

    Bleeding risk with AZD6140, a reversible P2Y12 receptor antagonist, vs. clopidogrel in patients undergoing coronary artery bypass grafting in the DISPERSE2 trial.

    International journal of clinical practice·2009
    Same author

    Targeting the polyamine biosynthetic enzymes: a promising approach to therapy of African sleeping sickness, Chagas' disease, and leishmaniasis.

    Amino acids·2007
    Same author

    Polyamine biosynthetic enzymes as drug targets in parasitic protozoa.

    Biochemical Society transactions·2003
    Same author

    The immediate and long-term effect of optimal balloon angioplasty on the absolute coronary blood flow velocity reserve. A subanalysis of the DEBATE study. Doppler Endpoints Balloon Angioplasty Trial Europe.

    European heart journal·2001
    Same journal

    Myosin expression in the developing ascidian embryo.

    The Journal of experimental zoology·2012
    Same journal

    Regulation in the chick allantois.

    The Journal of experimental zoology·2010
    Same journal

    A serologic study of conjugation in Paramecium bursaria.

    The Journal of experimental zoology·2010
    Same journal

    Studies on flagellates from domesticated birds; the behavior of Trichomonas gallinarum in culture.

    The Journal of experimental zoology·2010
    Same journal

    Experiments on the formed inclusions of the amphibian egg; the effect of pH and electrolytes on yolk and lipochondria.

    The Journal of experimental zoology·2010
    Same journal

    Production of strophosomy in the chick embryo by local applications of colchicine.

    The Journal of experimental zoology·2010
    See all related articles

    Polyamines and their synthesis enzymes, L-ornithine decarboxylase (ODC) and S-adenosyl-L-methionine decarboxylase (SAMDC), peak during key chick embryo development stages. Enzyme activity and polyamine levels correlate with organ growth rates.

    Area of Science:

    • Developmental Biology
    • Biochemistry

    Background:

    • Polyamines are essential for cell growth and differentiation.
    • Understanding polyamine synthesis regulation is crucial for developmental studies.

    Purpose of the Study:

    • To measure polyamine synthesis enzyme activities and polyamine concentrations during chick embryo development.
    • To correlate these with specific developmental stages and organ growth.

    Main Methods:

    • Enzyme activity assays for L-ornithine decarboxylase (ODC) and S-adenosyl-L-methionine decarboxylase (SAMDC).
    • Quantification of polyamines (putrescine, spermidine, spermine) using biochemical methods.
    • Analysis across various developmental time points from laying to hatching.

    Main Results:

    Related Experiment Videos

    • Major peaks in ODC, SAMDC activities, and polyamine concentrations observed at 15h (gastrulation), 23-30h (early organogenesis), days 4-5 (mid-organogenesis), and days 12-17 (maturation).
    • Higher ODC activity and polyamine levels in the head versus trunk region at 4.5 days.
    • Peak enzyme activities in lung, intestine, and kidney at 14 days, correlating positively with organ growth rates.

    Conclusions:

    • Polyamine metabolism is tightly regulated during chick embryogenesis, with distinct peaks corresponding to critical developmental phases.
    • Regional differences in polyamine synthesis exist, particularly between head and trunk regions.
    • Enzyme activities and polyamine levels are linked to organ growth and maturation, highlighting their importance in embryonic development.