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Direct demonstration of murine thymus-dependent cell surface endogenous immunoglobin
Summary
Chicken antibodies detect surface immunoglobulin (Ig) on mouse T cells and lymphomas, unlike mammalian antibodies. This finding reveals a distinct kappa chain determinant on T cell surface Ig, crucial for understanding T cell biology.
Area of Science:
- Immunology
- Cell Biology
- Oncology
Background:
- Mammalian antisera fail to detect surface immunoglobulin (Ig) on thymus-dependent (T) lymphoma cells.
- This limitation hinders the study of T cell surface markers and their role in lymphoma.
Purpose of the Study:
- To investigate the potential of non-mammalian antibodies for detecting surface Ig on T cells and lymphomas.
- To characterize the antigenic determinants recognized by these antibodies on T cell surface Ig.
Main Methods:
- Production and purification of chicken antibodies against mouse IgG (Fab)2 fragment.
- Immunofluorescence analysis of murine T cells and T lymphoma cells using chicken antibodies.
- Adsorption studies with myeloma-derived kappa chains to identify antigenic targets.
Main Results:
- Chicken antibodies strongly detected surface Ig on murine T cells and T lymphoma cells, unlike mammalian antisera.
- The detected surface Ig exhibited capping and shedding, indicating endogenous synthesis.
- Nonlymphoid myeloid tumor cells did not bind the chicken antibodies, despite Fc receptor expression.
- Binding of chicken antibodies was abolished by adsorption with kappa chains, suggesting recognition of a kappa determinant.
Conclusions:
- T lymphocytes and T lymphoma cells express and synthesize surface Ig.
- Chicken antibodies recognize a kappa antigenic determinant on T cell surface Ig that differs from those detected by mammalian antibodies.
- This distinct determinant may explain the differential detectability of surface Ig on T cells.