The proteins and RNAs specified by Clo Mor virus, a Scottish Nairovirus

The proteins and RNAs of Clo Mor virus have been analysed. Virus-specific proteins in infected cells had previously been identified by isotopic labelling and radioimmunoprecipitation; these were three glycoproteins (mol. wt. 115K, 90K and 80K) and an unglycosylated nucleocapsid (N) protein (50K). We have performed pulse-chase experiments which indicated that the 115K protein is processed to give the 90K and 80K proteins, while a 45K protein was detected in released virions after prolonged chase. Translation in vitro of mRNA extracted from Clo Mor virus-infected cells resolved only the N protein. Three species of RNA were extracted from Clo Mor virus intracellular nucleocapsids and have been designated L (11000 to 13000 bases), M (6300 bases) and S (1900 bases). The processing of viral proteins and the sizes of RNAs are characteristic of the Nairovirus genus of the family Bunyaviridae.