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Intermediate filaments in meningiomas.

W C Halliday, H Yeger, G F Duwe

    Journal of Neuropathology and Experimental Neurology
    |November 1, 1985
    PubMed
    Summary
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    Meningioma cells contain intermediate filaments (IF). This study found vimentin is the primary IF in most meningioma types, excluding angioblastic variants, confirmed by advanced protein analysis.

    Area of Science:

    • Neuroscience
    • Oncology
    • Cell Biology

    Background:

    • Intermediate filaments (IF) are crucial cytoskeletal components.
    • Electron microscopy reveals 10 nm IF in meningioma cell cytoplasm.
    • Histological variants of meningioma include transitional, syncytial, fibroblastic, and angioblastic types.

    Purpose of the Study:

    • To identify the specific intermediate filaments (IF) present in various meningioma subtypes.
    • To determine if cytokeratins or vimentin are the predominant IF in meningiomas.

    Main Methods:

    • Immunohistochemical staining for cytokeratins and vimentin.
    • Two-dimensional (2-D) gel electrophoresis.
    • Immunoblot analysis on 16 meningioma samples (13 surgical, 3 autopsy).

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    Main Results:

    • All meningiomas tested negative for cytokeratins.
    • All meningiomas showed positive staining for vimentin using monoclonal antibodies.
    • 2-D gel electrophoresis and immunoblotting confirmed vimentin and its breakdown products as the sole IF detected.

    Conclusions:

    • Vimentin is the principal intermediate filament (IF) found in transitional, syncytial, and fibroblastic meningiomas.
    • This finding clarifies the cytoskeletal composition of common meningioma subtypes.
    • Excludes cytokeratins as significant IF components in these meningiomas.