Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Determination of Crystal Structures01:29

Determination of Crystal Structures

139
In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...
139

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mesoglea biogenesis reveals a cryptic aboral valve for pressure regulation in cnidarian morphogenesis.

Science advances·2026
Same author

Radiation-induced peroxide rupture and its temperature-dependent repair probed by homogeneous X-ray irradiation.

Acta crystallographica. Section D, Structural biology·2026
Same author

Radiation damage in sub-Ångström resolution macromolecular crystallography: a low-dose study.

Acta crystallographica. Section D, Structural biology·2026
Same author

CryoRhodopsins: A comprehensive characterization of a group of microbial rhodopsins from cold environments.

Science advances·2025
Same author

Author Correction: DNA-guided transcription factor interactions extend human gene regulatory code.

Nature·2025
Same author

Proteorhodopsin insights into the molecular mechanism of vectorial proton transport.

Science advances·2025
Same journal

Scotty: lattice coincidences in the Protein Data Bank.

Acta crystallographica. Section D, Structural biology·2026
Same journal

Scotty: lattice coincidences for macromolecular crystallographic phasing.

Acta crystallographica. Section D, Structural biology·2026
Same journal

Miroslav Z. Papiz (1955-2026).

Acta crystallographica. Section D, Structural biology·2026
Same journal

Structural basis of regioselective double halogenation of the β-carboline tryptoline by the single-component halogenase AetF.

Acta crystallographica. Section D, Structural biology·2026
Same journal

Simulating neutron protein crystallography experiments: applications to the development of the NMX instrument at ESS.

Acta crystallographica. Section D, Structural biology·2026
Same journal

Molecular architecture of the human citrate synthase-malate dehydrogenase 2 metabolon.

Acta crystallographica. Section D, Structural biology·2026
See all related articles

Related Experiment Video

Updated: May 6, 2026

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect
06:42

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

Published on: March 19, 2019

5.9K

Enhanced capabilities for multi-crystal data collection based on double mesh scans.

Igor Melnikov1, Olof Svensson1, Gleb Bourenkov2

  • 1European Synchrotron Radiation Facility, BP 220, 38043 Grenoble, France.

Acta Crystallographica. Section D, Structural Biology
|June 26, 2025
PubMed
Summary
This summary is machine-generated.

The upgraded DoubleMesh&Collect method uses two X-ray mesh scans for precise 3D crystal positioning and quality assessment in macromolecular crystallography, improving data collection efficiency.

Keywords:
DozorDozor-m2ResheteauX-ray mesh raster scansdata-collection strategiesmacromolecular crystallographysample localization

More Related Videos

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules
07:11

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules

Published on: March 22, 2019

7.0K
Fixed Target Serial Data Collection at Diamond Light Source
06:19

Fixed Target Serial Data Collection at Diamond Light Source

Published on: February 26, 2021

3.4K

Related Experiment Videos

Last Updated: May 6, 2026

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect
06:42

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

Published on: March 19, 2019

5.9K
Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules
07:11

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules

Published on: March 22, 2019

7.0K
Fixed Target Serial Data Collection at Diamond Light Source
06:19

Fixed Target Serial Data Collection at Diamond Light Source

Published on: February 26, 2021

3.4K

Area of Science:

  • Crystallography
  • Structural Biology
  • X-ray Diffraction

Background:

  • Two-dimensional X-ray mesh scans are standard for locating sample positions in macromolecular crystallography.
  • The Mesh&Collect method utilizes single mesh scans to collect diffraction data from multiple crystals.
  • Previous methods had limitations in accurately determining crystal positions and dimensions.

Purpose of the Study:

  • To present an enhanced DoubleMesh&Collect method for improved crystal characterization.
  • To enable more accurate determination of 3D crystal centers and dimensions.
  • To optimize diffraction data collection strategies in macromolecular crystallography.

Main Methods:

  • Utilizing two X-ray mesh scans, typically at orthogonal orientations.
  • Employing the Dozor, Dozor-m2, and Resheteau software for automated analysis.
  • Analyzing diffraction images from dual mesh scans to determine crystal parameters.

Main Results:

  • Demonstrated accurate determination of 3D crystal center positions.
  • Provided improved estimates of crystal dimensions and diffraction qualities.
  • Successfully applied the method to various cases, including room-temperature studies.

Conclusions:

  • The DoubleMesh&Collect method offers superior accuracy over single mesh scans.
  • This upgraded method enhances the efficiency and reliability of macromolecular crystallography experiments.
  • Further discussion addresses the cartography of large crystals within the scanned area.