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Updated: Sep 18, 2025

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

MEBOCOST maps metabolite-mediated intercellular communications using single-cell RNA-seq.

Rongbin Zheng^1,2, Yang Zhang^3,4, Tadataka Tsuji^3,4

¹Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, United States.

Nucleic Acids Research

|June 26, 2025

Summary

This summary is machine-generated.

MEBOCOST is a new algorithm for detecting metabolite-mediated cell-cell communication (mCCC) using single-cell RNA sequencing and metabolic modeling. It identifies key mCCC interactions in obesity and adipocyte differentiation, advancing our understanding of metabolic regulation.

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Last Updated: Sep 18, 2025

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Published on: January 9, 2020

Area of Science:

Metabolomics
Systems Biology
Single-cell analysis

Background:

Cell-cell communication (CCC) is vital for tissue function.
Existing methods often miss metabolite-mediated CCC (mCCC).
Adapting protein-focused methods for mCCC is difficult.

Purpose of the Study:

To develop a computational tool for detecting mCCC.
To analyze mCCC in human obesity and mouse brown adipose tissue.
To identify novel mCCC pathways regulating adipocyte differentiation.

Main Methods:

Developed MEBOCOST algorithm integrating scRNA-seq and metabolic flux balance analysis.
Validated MEBOCOST using simulation, spatial, CRISPR screen, and clinical data.
Applied MEBOCOST to human white adipose tissue and mouse brown adipose tissue datasets.

Main Results:

MEBOCOST demonstrated robust detection of significant mCCC events.
Identified macrophages as major sources of mCCC reprogramming in obese patients.
Discovered a novel glutamine-mediated endothelial-to-adipocyte communication regulating adipocyte differentiation.

Conclusions:

MEBOCOST is a powerful tool for studying mCCC in various biological contexts.
Provides insights into metabolic dysregulation in obesity.
Facilitates the discovery and experimental verification of novel mCCC mechanisms.