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Published on: April 5, 2019
Investigation on Chiral Amino Acids Enantiomeric Excess With Cyclodextrin Inclusion Complex Ions Assisted
Simin Zhang1, Xiangfeng Chen1,2, T-Y Lui1
1Department of Chemistry, The Chinese University of Hong Kong, Hong Kong, Special Administrative Region, People's Republic of China.
None:
Development of a rapid, inexpensive, and specific method for chiral amino acids (AAs) identification and separation is challenging owing to their similar properties. In this work, a chiral AAs separation method was developed by analyzing the AAs-cyclodextrin (CD) host-guest complex in the CaptiveSpray-DMS-MS system. Compared with β- and γ-CDs, α-CD was revealed to provide the best differentiation performance. The optimized conditions enabled the analysis of six pairs of respective AA-α-CD complexes within several minutes. Ionograms obtained using this strategy effectively determined the enantiomeric ratio of chiral AAs, as illustrated by the strong linearity (R2 = 0.996) between peak abundance ratios and molar ratios of L-serine and D-serine. This method does not require the use of metal ions, and the single peak shape in ionograms of chiral AA-α-CD complex ions simplifies data interpretation.
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