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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Related Experiment Video

Updated: Sep 8, 2025

Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering

Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering

Published on: April 21, 2023

Improved genomic DNA extraction from citrus species using a modified CTAB method.

Komaljeet Gill¹, Shivanti Negi², Pankaj Kumar³

¹Department of Biotechnology, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, Himachal Pradesh, India. singh.komal2111@gmail.com.

Molecular Biology Reports

|June 26, 2025

Summary

This study optimized a CTAB DNA extraction protocol for citrus, yielding high-purity genomic DNA. The improved method significantly increases DNA yield and quality for downstream applications like PCR and sequencing.

Keywords:

CTAB method Citrus DNA isolation DNA quality DNA yield Gel electrophoresis Statistical analysis

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Last Updated: Sep 8, 2025

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Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

Published on: November 10, 2009

Area of Science:

Plant Molecular Biology
Biotechnology
Genomics

Background:

Citrus DNA extraction is challenging due to polysaccharides and polyphenols.
These compounds inhibit DNA yield and purity.
A robust protocol is needed for citrus genetic studies.

Purpose of the Study:

To optimize a CTAB-based protocol for efficient genomic DNA isolation from citrus leaves.
To improve DNA yield and purity compared to conventional methods.
To provide a reliable method for citrus genetic research.

Main Methods:

Optimized CTAB buffer with β-mercaptoethanol and PVP.
Phenol:chloroform:isoamyl alcohol extraction for contaminant removal.
RNase A treatment and isopropanol precipitation for DNA isolation.

Main Results:

High-molecular-weight DNA obtained with A260/280 ratios of 1.78-1.98.
Significantly higher DNA yield and band intensity compared to conventional methods (p < 0.001).
Intact DNA with minimal RNA contamination confirmed by gel electrophoresis.

Conclusions:

The optimized CTAB protocol offers a simple, cost-effective, and reproducible method.
It yields high-quality genomic DNA suitable for PCR, genotyping, and sequencing.
This protocol enhances citrus genetic research capabilities.