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Related Concept Videos

Human Virome01:26

Human Virome

The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible only with...

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Comparative wastewater virome analysis with different enrichment methods.

Matthew Thornton1, Gabriela Eder2, Fabian Amman3

  • 1Department of Hygiene and Applied Immunology, Medical University of Vienna, Kinderspitalgasse 15, Vienna 1090, Austria.

Water Research
|June 26, 2025
PubMed
Summary

Polyethylene glycol precipitation (PEG) and vacuum-based direct capture (VDC) are superior methods for concentrating diverse viruses in wastewater. These techniques enhance detection and genomic analysis for public health surveillance.

Keywords:
ConcentrationEnrichmentPCRSequencingViromeVirusWastewater surveillanceWastewater-based epidemiology

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Area of Science:

  • Environmental microbiology
  • Public health virology
  • Molecular diagnostics

Background:

  • Wastewater-based epidemiology (WBE) relies on sensitive virus detection.
  • Concentrating virus particles is essential for accurate WBE.
  • Evaluating different concentration methods is crucial for optimizing WBE.

Purpose of the Study:

  • To compare five virion concentration techniques for WBE.
  • To assess methods for PCR detection and metagenomic sequencing.
  • To identify optimal methods for capturing diverse viral taxa from wastewater.

Main Methods:

  • Polyethylene glycol precipitation (PEG)
  • Vacuum-based direct capture (VDC)
  • Ultrafiltration (UF)
  • NanoTrap® (NT)
  • Membrane adsorption (MEM)

Main Results:

  • PEG and VDC demonstrated superior enrichment rates and reproducibility.
  • These methods excelled in species detection and nucleotide diversity.
  • Variable concentration efficiencies were observed across methods and viral groups.

Conclusions:

  • PEG and VDC are equally effective for broad viral detection and enrichment in WBE.
  • These methods enhance genomic information potential and reduce WBE blind spots.
  • The findings guide protocol selection for future public health surveillance of viral threats.