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Updated: Sep 18, 2025

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
Published on: July 23, 2014
Transcriptome analysis reveals insights into regulatory networks of prickle formation in Rosa multiflora and the role
Wan Zhu1, Zhe Zhang2, Junjie Wu1
1National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China; The Institute of Flowers Research, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.
Abstract:
Roses are among the most important ornamental/commercial plants worldwide. However, prickles on rose stems present difficulties in field management as well as inconveniences during harvesting and postharvest processes. The spatiotemporal dynamics and regulatory network of prickle formation remain unclear. We report a temporally resolved transcriptome obtained from prickle samples covering the entire development period of Rosa multiflora and a comparative transcriptome of R. multiflora and its prickleless variant. Joint analysis was conducted on two sets of RNA-seq data, and a total of 700 prickle-related genes were divided into four categories corresponding to the positive/negative regulation of prickle initiation and development. Among these genes, RmNAC43 was coexpressed with three laccase genes, and its expression increased sharply with prickle development. A heterologous expression system suggested that RmNAC43 participates in lignin deposition partly by regulating the laccase gene during prickle hardening. Collectively, these results provide novel insights into molecular-level biological events during prickle formation and constitute potentially valuable resources for future genetic improvements in rose and other prickle-bearing plants.
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