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Updated: May 7, 2026

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples
Published on: July 16, 2017
Variations in the Structure and Composition of Soil Microbial Communities of Different Forests in the Daxing'anling
Han Qu1, Mingyu Wang1, Xiangyu Meng1
1Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, China.
Abstract:
Soil microorganisms are crucial in global biogeochemical cycles, impacting ecosystems' energy flows and material cycling. This study, via high-throughput sequencing in four forests-the original Larix gmelinii (Rupr.) Kuzen. forest (LG), the conifer-broad-leaved mixed Pinus sylvestris var. mongolica Litv. forest (PS), the original pure Betula platyphylla Sukaczev forest (BP), and the original pure Populus L. forest (PL) in Shuanghe National Nature Reserve, Daxing'anling mountains-explored soil microbial community structures and diversities. The results indicated that the BP and PL forests had the lowest soil bacterial ACE and Chao1 indices, and the original pure birch forest's Shannon index was higher than that of the poplar forest. The soil's fungal Chao1 index of the birch forest was higher than that of the larch forests. Bradyrhizobium and Roseiarcus were the dominant soil bacterial genera; the dominant soil fungal genera were Podila, Russula, and Sebacina. RDA and mantel analyses indicated that soil microbial community structures varied across forest types mainly because of the effective phosphorous and pH levels, soil's total nitrogen level, and available phosphorus level. This study offers a scientific foundation for cold-temperate-forest ecosystem management regarding soil microbial diversity and community structural changes in different forest types.
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