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FpFumB Is Required for Basic Biological Processes and Virulence in Fusarium proliferatum by Modulating DNA Repair

Yizhou Gao1, Haibo Li1, Yong Liu1

  • 1School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China.

Microorganisms
|June 27, 2025
PubMed

Insights

The fumarase enzyme FpFumB is crucial for Fusarium proliferatum growth, sporulation, and virulence. It also interacts with DNA repair protein FpSae2, impacting DNA damage response and pathogenicity.

Area of Science:

  • Biochemistry
  • Mycology
  • Plant Pathology

Background:

  • Fumarase is essential for the tricarboxylic acid cycle.
  • The role of fumarase in plant pathogenic fungi like Fusarium proliferatum is not well-understood.

Purpose of the Study:

  • To investigate the function of fumarase genes in Fusarium proliferatum.
  • To elucidate the role of FpFumB in fungal growth, stress tolerance, DNA repair, and virulence.

Main Methods:

  • Gene deletion and site-directed mutagenesis of fumarase genes.
  • Transcriptomic analysis to identify affected metabolic pathways.
  • Protein interaction assays to identify FpFumB interacting partners.

Main Results:

  • Deletion of FpFumB impaired fungal growth, sporulation, stress tolerance, and virulence.
  • FpFumB interacts with the DNA repair protein FpSae2.
  • Mutants lacking FpFumB or FpSae2 showed altered DNA damage sensitivity and reduced virulence.

Conclusions:

  • FpFumB is a key regulator of essential biological processes, DNA repair, and pathogenicity in Fusarium proliferatum.
  • FpFumB modulates virulence and DNA repair through its interaction with FpSae2.
Keywords:
DNA damage repairFusarium proliferatumTCA cyclefumarasepathogenicitystress response

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