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Updated: Sep 18, 2025

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

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Topical Application of RNAi Therapy Using Surface-Modified Liposomes for Treating Retinal-Vein Occlusion.

Taishi Shiratori¹, Takaaki Ito¹, Anri Nishinaka²

¹Laboratory of Pharmaceutical Engineering, Gifu Pharmaceutical University, 1-25-4 Daigaku-Nishi, Gifu 501-1196, Japan.

Molecules (Basel, Switzerland)

|June 27, 2025

Summary

New eye drop technology uses PnkRNA-loaded liposomes for retinal disease treatment. This novel nucleic acid delivery system offers a safer, simpler alternative to intravitreal injections, improving therapeutic efficacy.

Keywords:

drug delivery eye drop nucleic acid retina retinal-vein occlusion thermoresponsive gel

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Last Updated: Sep 18, 2025

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Intravitreal Injections in the Ovine Eye

Intravitreal Injections in the Ovine Eye

Published on: July 5, 2022

Area of Science:

Ophthalmology
Drug Delivery
Biotechnology

Background:

Retinal diseases cause significant vision loss and are a major health burden.
Current treatments, like antibody and nucleic acid therapies, require intravitreal injection, posing challenges.
There is a need for safer, more convenient methods for retinal drug delivery.

Purpose of the Study:

To develop a novel eye drop formulation for treating retinal diseases.
To evaluate the efficacy of PnkRNA-loaded liposomes for retinal drug delivery.
To improve sustained drug release and therapeutic outcomes for retinal conditions.

Main Methods:

Formulation of PnkRNA (a single-stranded RNA nucleic acid) loaded into liposomes.
Evaluation of liposomal delivery and therapeutic effects in a mouse retinal-vein occlusion model.
Incorporation of liposomes into thermoresponsive gels for sustained release and assessment of cationic modification for enhanced delivery.

Main Results:

PnkRNA-loaded liposomes effectively delivered retinal drugs and inhibited retinal thickening.
Cationic modification of liposomes enhanced nucleic acid delivery and therapeutic efficacy.
Thermoresponsive gel formulation provided sustained retinal delivery and superior efficacy compared to eye drops.

Conclusions:

Nucleic acid-based eye drops represent a significant advancement in retinal drug delivery.
This technology offers a safe, simple, and effective treatment option for retinal diseases.
Sustained release formulations improve therapeutic outcomes and reduce administration frequency.