Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Methods of Classification and Identification

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...

Automated Microbial Diagnostics

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Integrative single-cell and spatial transcriptomic analysis reveals that ATG13 enriched in cancer-associated fibroblasts is associated with the suppression of ferroptosis in colorectal cancer.

Discover oncology·2026

Same author

UCTLFANet: a low-rank fine-tuning model for microalgae image segmentation.

Scientific reports·2026

Same author

Study on the Protective Efficacy of the Japanese Encephalitis Live Attenuated Vaccine SA14-14-2 Against Newly Isolated Genotype I Japanese Encephalitis Viruses.

Viruses·2026

Same author

Genomic epidemiology of the 2025 mpox epidemic in Sierra Leone.

Nature medicine·2026

Same author

A modular protein-nucleic acid nanodevice for non-genetic engineering of mesenchymal stem cells to target pathological collagen in liver fibrosis.

Journal of nanobiotechnology·2026

Same author

Characterisation of the First Complete Dengue Genome in Sierra Leone.

Viruses·2026

Search research articles

Related Experiment Video

Updated: May 7, 2026

Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved

Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved

Published on: August 2, 2011

Facilitating Cross-border Viral Sequencing Through Nucleic Acid Sample Transport Using Dry Cards.

Lili Wang^1,2, Qikai Yin¹, Alie Brima Tia³

¹National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

|June 27, 2025

Summary

Flinders Technology Associates (FTA) cards enable virus genomic sequencing from amplified nucleic acid samples in resource-limited settings. This method enhances infectious disease monitoring and outbreak response capabilities in underdeveloped areas.

Keywords:

dry cards sample transport stability viral sequencing

More Related Videos

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Published on: July 2, 2016

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

Related Experiment Videos

Last Updated: May 7, 2026

Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved

Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved

Published on: August 2, 2011

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Published on: July 2, 2016

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

Area of Science:

Virology
Genomics
Public Health

Background:

Developing countries often lack advanced sequencing infrastructure for infectious disease surveillance.
A novel nucleic acid sample transportation method is needed for virus genomic sequencing in resource-limited areas.

Purpose of the Study:

To evaluate the efficacy of Flinders Technology Associates (FTA) cards for transporting amplified whole-genome DNA of SARS-CoV-2.
To assess the feasibility of virus genomic sequencing from samples transported using FTA cards in resource-limited settings.

Main Methods:

120 SARS-CoV-2 positive nasopharyngeal swab samples were collected in Sierra Leone.
Nucleic acid extraction and whole-genome amplification were performed locally.
Amplified DNA was applied to FTA Elute cards for room temperature shipment and subsequent elution and sequencing.

Main Results:

The FTA card method demonstrated a 9.6% amplicon recovery rate, sufficient for viral genome sequencing.
86 (71.7%) high-quality SARS-CoV-2 genomic sequences were obtained, with most exceeding 100X depth.
Co-circulation of Delta, Omicron, and B.1 lineages was identified; samples with Ct ≤ 27 yielded effective sequences (73.6%).

Conclusions:

Transportation of amplified nucleic acid samples on FTA cards facilitates virus genomic sequencing in resource-limited regions.
This approach can enhance local virus surveillance and outbreak response.
Further optimization may improve sequence recovery rates, increasing sequencing accessibility in underdeveloped areas.