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Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
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Medicago Sativa Stems-A Multi-Output Integrated Biorefinery Approach.

Adrian Cătălin Puițel1, George Bârjoveanu1, Cătălin Dumitrel Balan1

  • 1Faculty of Chemical Engineering and Environmental Protection "Cristofor Simionescu", Technical University "Gheorghe Asachi" Iasi, Bd. Prof. Dimitrie Mangeron, No. 73, 700050 Iaşi, Romania.

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Summary
This summary is machine-generated.

Field-stored alfalfa (Medicago sativa) can be processed in a biorefinery to extract valuable protein and cellulose. Alfalfa pulp improves the strength of recycled paper, supporting circular economy principles.

Keywords:
alfalfabiorefinerypapermaking fibersprotein extractspulpingstructural carbohydrateswaste biomass

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Area of Science:

  • Biomass valorization
  • Sustainable materials science
  • Circular economy

Background:

  • Field-stored one-year-old alfalfa (Medicago sativa) represents an underutilized biomass resource.
  • Developing multi-output biorefineries is crucial for sustainable resource management and waste reduction.

Purpose of the Study:

  • To investigate the fractionation of alfalfa biomass into crude protein, polysaccharides, lignin, and cellulose.
  • To evaluate the suitability of extracted cellulose for papermaking applications.

Main Methods:

  • Biomass pretreatment using water, alkaline buffer, and NaOH solutions.
  • Soda pulping under varying severity conditions (20% NaOH, 160 °C, 60 min).
  • Chemical characterization of solid and liquid fractions using FTIR, HPLC, and standard methods.

Main Results:

  • Water pretreatment achieved over 40% protein content in precipitated fractions.
  • Harshest pulping conditions yielded cellulose-rich pulp with high glucan content.
  • Alfalfa pulp blended with old corrugated cardboard (OCC) enhanced tensile (35%) and burst (70%) strength.

Conclusions:

  • Alfalfa biomass can be effectively fractionated into valuable components.
  • Alfalfa-derived pulp shows significant potential for enhancing papermaking properties.
  • This approach supports the valorization of agricultural residues and promotes circular economy principles.