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Related Experiment Videos

Kinetics of endothelial cell seeding.

J E Rosenman, R F Kempczinski, W H Pearce

    Journal of Vascular Surgery
    |November 1, 1985
    PubMed
    Summary

    Determining the optimal number of endothelial cells for seeding Dacron grafts is crucial. This study found that approximately 2.72 X 10^4 cells are adequate to seed 12.5 cm^2 of graft, improving graft patency.

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    Area of Science:

    • Biomaterials Science
    • Vascular Surgery
    • Cell Biology

    Background:

    • Endothelial cell seeding enhances Dacron graft patency and promotes a complete endothelial surface.
    • The optimal cell density for seeding small-diameter grafts remains undetermined.

    Purpose of the Study:

    • To determine the ideal number of endothelial cells required for seeding Dacron grafts.
    • To quantify initial cell adherence and subsequent cell loss from seeded grafts over time.

    Main Methods:

    • Autogenous endothelial cells were labeled with indium 111-oxine.
    • Cells were seeded onto 10 cm long, 4 mm I.D. porous polytetrafluoroethylene grafts.
    • Graft radioactivity was monitored using a gamma camera for up to 72 hours post-implantation in a canine carotid artery model.

    Main Results:

    • A mean of 19.8% of harvested cells adhered initially to the grafts.
    • Rapid cell loss occurred within the first 30 minutes after flow restoration (mean 70.2% remaining).
    • A constant cell loss rate of 3.7% per hour was observed between 30 minutes and 24 hours (r = -0.922, p < 0.001).
    • Beyond 24 hours, cell loss was insignificant.

    Conclusions:

    • Approximately 2.72 X 10^4 endothelial cells (4.4% of harvested) are sufficient to seed 12.5 cm^2 of graft.
    • This finding provides a quantitative basis for optimizing endothelial cell seeding protocols for vascular grafts.

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