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Related Experiment Video

Updated: Sep 18, 2025

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
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3D Bioprinted Renal Constructs Using Kidney-Specific ECM Bioink System on Kidney Regeneration.

Gabriel Carreno-Caleano1, Mohamed Ali1,2, James J Yoo1

  • 1Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston-Salem, NC, 27157, USA.

Advanced Healthcare Materials
|June 27, 2025
PubMed
Summary

Researchers developed a kidney-specific bioink from decellularized extracellular matrix (kdECMMA) for 3D bioprinting. This innovative bioink supports renal cell growth and maturation, showing promise for regenerating damaged kidneys.

Keywords:
bioinkbioprintingextracellular matrixkidneyrenal regeneration

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Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Tissue Engineering

Background:

  • End-stage chronic kidney disease (CKD) is irreversible, with limited treatment options like dialysis and transplantation.
  • 3D bioprinting offers a potential strategy for engineering functional renal constructs to repair kidney damage.
  • Developing a kidney-specific bioink is crucial for supporting renal cell organization, maturation, and function.

Purpose of the Study:

  • To utilize a kidney-derived decellularized extracellular matrix bioink (kdECMMA) for renal tissue bioprinting.
  • To assess the printability, structural integrity, and biological characteristics of the kdECMMA bioink.
  • To evaluate the in vitro and in vivo feasibility of kdECMMA-based bioprinted renal constructs for kidney regeneration.

Main Methods:

  • Preparation and characterization of a kidney-derived decellularized extracellular matrix bioink (kdECMMA).
  • Encapsulation of human kidney cells within kdECMMA for 3D bioprinting of renal constructs.
  • In vitro assessment of cell viability and maturation within bioprinted constructs.
  • In vivo implantation of bioprinted constructs into nude rats to evaluate renal tissue formation and integration.

Main Results:

  • The kdECMMA bioink demonstrated excellent printability, structural integrity, and biological properties.
  • Encapsulated human kidney cells showed high viability and progressive maturation within the bioprinted constructs.
  • Implanted constructs exhibited newly formed glomerular- and tubular-like structures with integrated human cells.
  • Cell-free kdECMMA constructs also promoted renal tissue formation, suggesting host progenitor cell recruitment.

Conclusions:

  • Kidney-derived decellularized extracellular matrix bioink (kdECMMA) is a viable material for renal tissue bioprinting.
  • kdECMMA supports human renal cell viability, maturation, and in vivo tissue regeneration.
  • This approach holds significant potential for engineering kidney-specific microenvironments to promote renal regeneration.