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Essential Minerals for Bone Health01:31

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The minerals contained in all of the food we consume are essential for our organ systems. However, certain essential minerals, such as calcium, phosphorus, magnesium, manganese, and fluoride, largely affect bone health.
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Updated: Sep 18, 2025

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
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Axin2 Deficiency Causes Hypomineralization and Delayed Tooth Development.

J Song1, B Kim1, J Na1

  • 1Graduate School of Medicine, Korea University, Ansan, Republic of Korea.

Journal of Dental Research
|June 27, 2025
PubMed
Summary
This summary is machine-generated.

Axin2 deficiency in zebrafish causes delayed tooth development and hypomineralization. This highlights the Wnt signaling pathway

Keywords:
anodobtiadentindentinogenesishypodontiaodontoblastszebrafish

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Area of Science:

  • Developmental Biology
  • Genetics
  • Oral Biology

Background:

  • The Wnt signaling pathway is critical for tooth development.
  • Axin2 is a negative regulator of Wnt signaling.

Purpose of the Study:

  • To investigate the role of Axin2 in zebrafish tooth development.
  • To analyze the effects of Axin2 deficiency on tooth formation and mineralization.

Main Methods:

  • Generated Axin2 knockout zebrafish using CRISPR/Cas9.
  • Utilized micro-CT, SEM, EDS, and qPCR for analysis.
  • Observed tooth development in a fluorescent transgenic line.

Main Results:

  • Axin2 deficiency led to reduced body length and delayed tooth initiation/morphogenesis.
  • Increased beta-catenin expression and decreased dentin elements (P, Ca, C, O) were observed.
  • Reduced expression of key genes involved in mineralization and bone formation (runx2a/b, sp7, scpp5, col1a1a/b).

Conclusions:

  • Loss of Axin2 function results in hypomineralization and delayed tooth development in zebrafish.
  • Spatiotemporal regulation of Wnt signaling is essential for odontoblast maturation.