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Tunable methacrylated decellularized heart matrix: a versatile scaffold for cardiac tissue engineering.

Valinteshley Pierre1, Douglas H Wu1,2, Chao Liu1

  • 1Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, United States.

Frontiers in Bioengineering and Biotechnology
|June 27, 2025
PubMed
Summary

This study enhances decellularized heart matrix (DHM) for cardiac tissue engineering. Modified DHM (DHMMA) offers tunable stiffness and improved mechanical properties, supporting cell survival and tissue regeneration applications.

Keywords:
biomaterialsdecellularized heart matrixmatrix protein releasemethacrylationultra-violet crosslinking

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Cardiovascular Research

Background:

  • Therapeutic tissue regeneration is crucial for treating heart failure and cardiovascular diseases, major global health concerns.
  • Decellularized heart matrix (DHM) shows potential for tissue engineering due to low immunogenicity and good biocompatibility.
  • A key limitation of DHM is its insufficient mechanical strength for biomedical applications.

Purpose of the Study:

  • To enhance the mechanical properties of decellularized heart matrix (DHM) for cardiac tissue engineering.
  • To develop a method for tuning the stiffness of DHM scaffolds.
  • To assess the suitability of the modified DHM for cell survival and microfabrication.

Main Methods:

  • Decellularized heart matrix (DHM) was functionalized with methacryloyl groups to create DHMMA.
  • UV-induced crosslinking was employed to enhance mechanical properties and tune stiffness.
  • Soft lithography was used to impart topographical features onto the DHMMA scaffolds.

Main Results:

  • Methacryloyl functionalization (DHMMA) significantly improved the mechanical properties of DHM.
  • Tunable stiffness was achieved by modulating the degree of methacryloyl substitution, UV exposure, and pH.
  • Crosslinked DHMMA maintained cell viability and supported cell orientation via topographical features.

Conclusions:

  • DHMMA presents a promising scaffold for cardiac tissue engineering with tunable mechanical properties.
  • The modified matrix supports cell survival and allows for microfabrication, addressing limitations of native DHM.
  • This approach offers a viable strategy for developing advanced biomaterials for cardiovascular regenerative medicine.