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Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
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Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Related Experiment Video

Updated: Sep 18, 2025

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL
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Nonlight-Driven Aggregation-Induced Emission Luminogens for Bioimaging and Theranostics.

Yong Tian1,2, Weigeng Huang1, Zhijia Sheng1,2

  • 1Center for AIE Research, Guangdong Provincial Key Laboratory of New Energy Materials Service Safety, Shenzhen University, Shenzhen 518060, China.

Chemical & Biomedical Imaging
|June 27, 2025
PubMed
Summary

Aggregation-induced emission luminogens (AIEgens) show promise for bioimaging. Unconventional excitation sources overcome limitations of traditional light, enhancing AIEgen applications in medicine.

Keywords:
X-rayaggregation-induced emissionchemiluminescencephototheranosticsultrasound

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Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Chemistry

Background:

  • Aggregation-induced emission luminogens (AIEgens) are advanced materials for optical imaging and theranostics.
  • Current AIEgen applications are limited by conventional light sources, causing poor tissue penetration and autofluorescence.

Purpose of the Study:

  • To explore unconventional excitation sources for AIEgens in biomedical applications.
  • To overcome limitations of traditional light excitation in bioimaging and theranostics.

Main Methods:

  • Reviewing fundamental principles of combining AIEgens with unconventional excitation.
  • Highlighting recent advancements in AIEgen bioimaging and theranostics using alternative excitation methods.

Main Results:

  • Unconventional sources (chemical, ultrasound, X-ray) offer effective alternatives to traditional light.
  • These methods circumvent drawbacks like limited penetration depth and background noise.

Conclusions:

  • Unconventional excitation significantly enhances AIEgen performance for biomedical uses.
  • Future research should focus on overcoming challenges to advance AIEgen-based diagnostics and therapeutics.