JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

Peroxynitrite Responsive Second Near-Infrared Cyanine Dye J‑Aggregate for Drug-Induced Hepatotoxicity Monitoring

  • 0State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Chemical & Biomedical Imaging +

|

June 27, 2025

|

June 27, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

A novel probe detects peroxynitrite (ONOO<sup>-</sup>), an early indicator of drug-induced liver injury. This advancement enables real-time <i>in vivo</i> monitoring of hepatotoxicity, overcoming limitations of current diagnostic methods.

Area Of Science

  • Biomedical Engineering
  • Chemical Biology
  • Medical Imaging

Background

  • Drug-induced hepatotoxicity is a significant clinical challenge.
  • Peroxynitrite (ONOO<sup>-</sup>) is an early biomarker for drug-induced liver injury.
  • Existing <i>in vivo</i> imaging methods for ONOO<sup>-</sup> are limited by autofluorescence and light scattering.

Purpose Of The Study

  • To develop a novel probe for sensitive and real-time detection of ONOO<sup>-</sup> <i>in vivo</i>.
  • To overcome the limitations of visible and NIR-I window probes for hepatotoxicity monitoring.

Main Methods

  • Synthesized a peroxynitrite (ONOO<sup>-</sup>) responsive cyanine dye, IR-1061 <i>J</i>-aggregate (<i>J</i><sub>IR-1061</sub>), with absorption in the NIR-IIb window (1580 nm).
  • Conjugated <i>J</i><sub>IR-1061</sub> with rare earth nanoparticles (RENPs) emitting at 1550 nm to create a dual-mode probe (RENPs-<i>J</i><sub>IR-1061</sub>).
  • Evaluated the probe's response to ONOO<sup>-</sup> and its efficacy for real-time hepatotoxicity monitoring <i>in vivo</i>.

Main Results

  • The developed probe, RENPs-<i>J</i><sub>IR-1061</sub>, exhibits a significant red shift (>500 nm) and operates in the NIR-IIb region.
  • The probe demonstrated a fast and sensitive response to ONOO<sup>-</sup>, indicated by activatable NIR-IIb fluorescence and photoacoustic signal changes.
  • Successfully applied for real-time <i>in vivo</i> monitoring of drug-induced hepatotoxicity.

Conclusions

  • RENPs-<i>J</i><sub>IR-1061</sub> is a promising dual-mode imaging probe for detecting ONOO<sup>-</sup>.
  • This probe facilitates real-time, unambiguous visualization of drug-induced hepatotoxicity <i>in vivo</i>.
  • Offers a potential new tool for early diagnosis and management of liver injury.

Keywords: