Related Experiment Video

Updated: Sep 17, 2025

Fully Endoscopic Mitral Valve Repair with Percutaneous Cannulation of Groin Vessels
08:12

Fully Endoscopic Mitral Valve Repair with Percutaneous Cannulation of Groin Vessels

Published on: May 26, 2023

1.8K

Rapid valve sterilization with meropenem plus ceftolozane/tazobactam combination therapy for Pseudomonas aeruginosa

Valliammai Alaguvel1, Anuj K Khetarpal1, Allen Jankeel1

  • 1Department of Pediatrics, University of California Irvine School of Medicine, Irvine, CA 92697, USA.

Jac-Antimicrobial Resistance
|June 27, 2025
PubMed
Abstract

Insights

This study shows that combining meropenem and ceftolozane/tazobactam effectively treated Pseudomonas aeruginosa prosthetic valve endocarditis. The combination demonstrated synergy in human blood and biofilm, offering a promising alternative for challenging infections.

Area of Science:

  • Infectious Diseases
  • Pharmacology
  • Microbiology

Background:

  • * Pseudomonas aeruginosa infective endocarditis (IE) is a severe condition with high treatment failure and mortality rates.
  • * Current therapies, including antipseudomonal β-lactams with aminoglycosides or fluoroquinolones, often lack efficacy and cause adverse effects.

Purpose of the Study:

  • * To report a case of P. aeruginosa prosthetic valve endocarditis successfully treated with meropenem and ceftolozane/tazobactam.
  • * To investigate the synergistic effects of this antibiotic combination in various in vitro and ex vivo models.

Main Methods:

  • * Case report of P. aeruginosa prosthetic valve endocarditis.
  • * In vitro assays: checkerboard, time-kill, human whole blood killing, and biofilm assays.
  • * Ex vivo model: simulated endocardial vegetation (SEV) model.

Main Results:

  • * Meropenem plus ceftolozane/tazobactam achieved microbiological clearance and facilitated cardiac surgery.
  • * Synergy was observed in human whole blood and biofilm assays, and modestly in the SEV model.
  • * Additivity was noted in checkerboard assays; synergy was absent in standard time-kill assays.

Conclusions:

  • * The combination of meropenem and ceftolozane/tazobactam shows promising synergistic activity in conditions mimicking the human body.
  • * This combination may offer a safer alternative for treating P. aeruginosa IE and stabilizing patients before surgery.
  • * Further clinical trials are warranted to confirm efficacy and safety in severe Pseudomonas infections.

More Related Videos

Transcatheter Pulmonary Valve Replacement from Autologous Pericardium with a Self-Expandable Nitinol Stent in an Adult Sheep Model05:31

Transcatheter Pulmonary Valve Replacement from Autologous Pericardium with a Self-Expandable Nitinol Stent in an Adult Sheep Model

Published on: June 8, 2022

3.0K
Development of a Polymicrobial Colony Biofilm Model to Test Antimicrobials in Cystic Fibrosis07:16

Development of a Polymicrobial Colony Biofilm Model to Test Antimicrobials in Cystic Fibrosis

Published on: September 20, 2024

1.2K

Related Concept Videos

Endocarditis III: Medical Management01:18

Endocarditis III: Medical Management

Infective endocarditis management involves a multifaceted approach encompassing infection prevention, lifestyle modifications, pharmacological therapy, and surgical management.Infection Prevention:Hand Hygiene: Thorough handwashing is crucial to prevent the spread of infection. Hand hygiene should be performed regularly, especially before and after using the restroom.Oral Hygiene: Good oral hygiene is essential. It includes brushing teeth immediately after waking up and before bed, flossing...
23
Endocarditis IV: Nursing Management01:29

Endocarditis IV: Nursing Management

Infective endocarditis (IE) is a chronic infection of the heart's endocardium, primarily affecting the heart valves. A detailed nursing assessment for a patient with IE involves collecting subjective and objective data to ensure an accurate diagnosis and timely intervention.Subjective DataThe nurse gathers information about the patient's symptoms and complaints during the subjective assessment. Patients with infective endocarditis often report non-specific symptoms that can mimic other...
40
Mitral Stenosis III: Medical Management01:26

Mitral Stenosis III: Medical Management

Mitral stenosis, a condition marked by the narrowing of the mitral valve, necessitates an integrated approach for effective management. This approach includes preventative measures, medical therapy, and surgical interventions to reduce symptoms and prevent complications.PreventionPrevention of mitral stenosis primarily focuses on reducing the incidence of bacterial infections, particularly streptococcal infections, which can lead to rheumatic fever and subsequent valvular damage. Timely...
28
Endocarditis I: Introduction01:25

Endocarditis I: Introduction

Introduction:Endocarditis is the infection of the endocardium, the inner lining of the heart and its valves. When the heart muscle is involved, the condition is termed myocarditis, while an infection of the outer lining is called pericarditis. Infective endocarditis (IE) primarily affects the endocardium, where pathogens adhere to the valves or lining, forming vegetation that can lead to severe complications. Infective endocarditis occurs when microorganisms, usually bacteria from other body...
32
Pneumonia IV: Management01:28

Pneumonia IV: Management

The treatment of pneumonia varies based on its severity and the causative pathogen. Here is a structured approach to managing pneumonia, integrating pharmaceutical and supportive care strategies.
Bacterial Pneumonia Treatment
For bacterial pneumonia, antibiotics serve as the cornerstone of therapy. Initial treatment often begins with empirical antibiotics, tailored to the anticipated causative organism and adjusted based on culture results. Key antibiotic choices include:
456
Acute Pyelonephritis II: Diagnostic Studies and Management01:28

Acute Pyelonephritis II: Diagnostic Studies and Management

Introduction:For diagnosing acute pyelonephritis, a comprehensive patient history is collected to identify symptoms such as dysuria, frequent or urgent urination, flank pain, or costovertebral angle (CVA) tenderness that may suggest a kidney infection.Physical ExaminationDuring the physical examination, CVA tenderness is assessed. This involves gentle percussion over the costovertebral angle, where tenderness often indicates a kidney infection.Diagnostic TestsUrinalysis: Used to identify white...
47