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Updated: Sep 17, 2025

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR
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The flexibility of SABRE, a new quantitative receptor function model, when fitting challenging concentration-effect

Barbara Olah1,2, Vera Tarjanyi2,3, Barbara Takacs3

  • 1Department of Orthodontics, Faculty of Dentistry, University of Debrecen, Debrecen, Hungary.

Frontiers in Pharmacology
|June 27, 2025
PubMed
Summary

The new Signal Amplification, Binding affinity, and Receptor-activation Efficacy (SABRE) model effectively analyzes receptor function data. It proves comparable to existing methods but requires diverse, high-quality data for reliable results.

Keywords:
SABRE modelatriumcurve fittingfurchgott methodinotropyoperational model

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Area of Science:

  • Pharmacology
  • Biophysics
  • Computational Biology

Background:

  • Quantitative receptor function models are crucial for understanding drug-receptor interactions.
  • Existing models like the operational model and Furchgott's method have limitations in fully characterizing receptor behavior.
  • The Signal Amplification, Binding affinity, and Receptor-activation Efficacy (SABRE) model offers a novel approach to receptor function analysis.

Purpose of the Study:

  • To evaluate the practical utility and capabilities of the SABRE model.
  • To compare the SABRE model's performance against established methods using concentration-effect data.
  • To identify the data requirements and fitting strategies for optimal SABRE model application.

Main Methods:

  • Utilized concentration-effect (E/c) data from a prior laboratory investigation.
  • Applied the SABRE model for quantitative analysis of receptor function.
  • Compared the SABRE model's results with those obtained from the operational model of agonism and Furchgott's method.

Main Results:

  • The SABRE model demonstrated comparable, and in some aspects superior, performance to existing models.
  • The model successfully determined key parameters like Kd and q from functional data.
  • Effective application of the SABRE model necessitates substantial, high-quality, and diverse experimental data.
  • Appropriate data fitting strategies are critical for achieving reliable outcomes.

Conclusions:

  • The SABRE model is a valuable tool for quantitative receptor function analysis, offering insights beyond traditional methods.
  • Its ability to differentiate receptor activation from post-receptorial signaling enhances its utility in simulating E/c curves.
  • While robust, the model's successful implementation depends on careful data acquisition and analysis strategies.