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Updated: Jul 17, 2026

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
Published on: August 19, 2012
New Click on the Block: Photocaged Hydroxylamine, an Addition to the Light-Activatable Bioorthogonal Toolbox
Krisztina Németh1, Flóra Horváth1, Ágnes Szatmári1
1MTA-HUN-REN RCNS Lendület "Momentum" Chemical Biology Research Group, Institute of Organic Chemistry, HUN-REN Research Centre for Natural Sciences, Magyar tudósok krt. 2, Budapest, H-1117, Hungary.
Abstract:
A new photoactivatable bioorthogonal function was added to the photoactivatable bioorthogonal toolbox by transiently disabling the bioorthogonal reactivity of the hydroxylamine moiety with a photolabile protecting group. The photocaged bioorthogonal function was further equipped with a HaloTag substrate to enable its use in live-cell labeling schemes. Light-controlled recovery of reactivity was demonstrated by bioorthogonal fluorescent labeling of intracellular proteins in live cells.

