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Related Experiment Video

Updated: Sep 17, 2025

Cellular Redox Profiling Using High-content Microscopy
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Optical Redox Imaging Predicts Post-Loading Cartilage Mitochondrial Membrane Potential.

Jingyi Wang1, Greta E Scheidt2, Corinne R Henak3,4,5

  • 1Department of Mechanical Engineering, University of Wisconsin-Madison, 3031 Mechanical Engineering Building, 1513 University Ave, Madison, WI, 53706, USA.

Annals of Biomedical Engineering
|June 27, 2025
PubMed
Summary
This summary is machine-generated.

Optical redox imaging (ORI) can predict mitochondrial membrane potential in cartilage without dyes. This method shows potential for evaluating cartilage redox balance and disease, offering a dye-free clinical tool.

Keywords:
CartilageMechanobiologyMitochondrial membrane potentialOptical redox imaging

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Area of Science:

  • Biomedical Engineering
  • Cellular Biology
  • Biophysics

Background:

  • Disrupted cellular redox balance is implicated in diseases like osteoarthritis.
  • Mitochondrial membrane potential serves as a redox balance indicator but often requires exogenous dyes.
  • Current dye-based methods have limitations for clinical translation.

Purpose of the Study:

  • To predict mitochondrial membrane potential changes in cartilage under mechanical loading using dye-free optical redox imaging (ORI).
  • To assess the impact of mechanical loading on ORI metrics.
  • To explore ORI as a potential clinical tool for assessing cartilage health.

Main Methods:

  • Porcine cartilage strips underwent tensile loading at different strain rates (1.00 s⁻¹ or 0.10 s⁻¹).
  • Optical redox imaging (ORI) was performed pre-loading, immediately post-loading, and 30 minutes post-loading.
  • Mitochondrial membrane potential was measured using fluorescent dye, and generalized linear mixed-effects models (GLMM) were used for analysis.

Main Results:

  • ORI metrics, loading rate, and loading conditions significantly predicted mitochondrial red/green ratio (average prediction difference of 7.07%).
  • Loading rate was a significant factor affecting ORI metrics.
  • The study demonstrated ORI's capability to predict dye-measured mitochondrial membrane potential.

Conclusions:

  • Optical redox imaging (ORI) can accurately predict mitochondrial membrane potential in cartilage.
  • ORI offers a promising dye-free approach for evaluating cartilage redox balance.
  • ORI has potential for development into a clinical tool for disease assessment.