Stimulating angiogenesis and post-ischemic tissue repair: Optimizing photobiomodulation parameters in vivo

Area of Science:

• Biomedical Engineering
• Regenerative Medicine
• Photomedicine

Background:

• Photobiomodulation (PBM) therapy uses light to stimulate cellular processes.
• Angiogenesis and tissue repair are critical for recovery from ischemic injuries.
• Optimizing PBM parameters is essential for maximizing therapeutic outcomes.

Purpose of the Study:

• To optimize photobiomodulation (PBM) parameters for enhanced angiogenesis and tissue repair.
• To evaluate the efficacy of PBM in preclinical models of vascularization and ischemia.
• To determine the impact of specific wavelengths and dosimetry on PBM's therapeutic effects.

Main Methods:

• PBM parameters were optimized using the chicken embryo chorioallantoic membrane (CAM) model.
• Pro-angiogenic effects were assessed via the aortic ring assay.
• Efficacy in hindlimb ischemia (HLI) was evaluated in mice, including light dosimetry and blood flow measurements.

Main Results:

• Optimized PBM significantly increased vascular formation in the CAM model.
• PBM demonstrated pro-angiogenic effects, promoting microvascular sprouting in aortic rings.
• Daily PBM at 652 nm improved blood flow recovery in the mouse HLI model by 14 days post-surgery.

Conclusions:

• Precise wavelength selection and dosimetry are crucial for effective PBM therapy.
• PBM enhances vascular remodeling and tissue repair, particularly as tissue oxygenation recovers.
• PBM represents a promising non-invasive strategy for treating ischemic conditions.