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Related Experiment Video

Updated: Sep 17, 2025

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Published on: October 7, 2016

Expression of Netrin-1 in the developing mouse heart.

Adrianna Matos-Nieves¹, Sarah C Greskovich¹, Talita Z Choudhury¹

¹Center for Cardiovascular Research, Abigail Wexner Research Institute, and The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA.

Gene Expression Patterns : GEP

|June 27, 2025

Summary

Netrin-1 is newly found in developing mouse hearts, particularly in left ventricular trabecular cardiomyocytes. This discovery suggests Netrin signaling may play a role in cardiac development.

Keywords:

Axon guidance signaling Heart development Netrin RNA in situ hybridization Trabecular myocardium

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Last Updated: Sep 17, 2025

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Published on: October 7, 2016

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

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Published on: July 12, 2022

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

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Published on: July 27, 2022

Area of Science:

Cardiovascular Biology
Developmental Biology
Molecular Signaling

Background:

Axon guidance pathways like Eph/ephrin, Semaphorin, and Slit/Robo are vital for cardiac development.
Netrin signaling is crucial for axon guidance but its role in heart development remains unexplored.

Purpose of the Study:

To investigate the role of Netrin signaling in the developing murine heart.
To describe the novel expression pattern of Netrin-1 in embryonic mouse hearts.

Main Methods:

Transcriptomic analysis of embryonic mouse hearts (E8.5-E14.5).
RNA in situ hybridization.
Utilized Netrin-1^Bgeo/+ reporter mice to track spatiotemporal expression.

Main Results:

Netrin-1 exhibits dynamic expression in the developing murine heart from E8.5 to E14.5.
Netrin-1 expression preferentially co-localizes with developing trabecular cardiomyocytes.
Highest Netrin-1 expression observed in the left ventricular trabecular myocardium, with lower levels in the right ventricle and atria.

Conclusions:

The identified expression pattern of Netrin-1 in the developing heart supports its potential role in cardiac development.
Further functional studies are required to elucidate the specific functions of Netrin signaling in the murine myocardium.