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Adiponectin Receptor Agonist Suppresses Human Colorectal Cancer

Mai Ly Thi Nguyen1, Lan Anh Bui2,3,4, Chi Pham2

  • 1Department of Biochemistry, Military Hospital 103, Hanoi, Vietnam.

Anticancer Research
|June 27, 2025
PubMed
Abstract

Insights

AdipoRon, an adiponectin receptor agonist, inhibits colorectal cancer (CRC) cell growth and migration by activating tumor suppressor pathways. This study highlights AdipoRon

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Colorectal cancer (CRC) remains a leading cause of cancer deaths globally, with limited therapeutic advancements.
  • Adiponectin signaling exhibits tumor-suppressive properties, making its pathway a potential therapeutic target for CRC.
  • AdipoRon, an adiponectin receptor agonist, offers a novel strategy to restore adiponectin signaling.

Purpose of the Study:

  • To investigate the anti-cancer effects of AdipoRon on colorectal cancer cells.
  • To evaluate AdipoRon's impact on cell proliferation, migration, apoptosis, and cell cycle.
  • To determine AdipoRon's effect on key signaling proteins like AMPKα and STAT3.

Main Methods:

  • In vitro assays including cell viability, crystal violet staining, migration, apoptosis, and cell cycle analysis.
  • Western blotting to assess protein expression levels of AMPKα, phosphorylated AMPKα (pAMPKα), and STAT3.

Main Results:

  • AdipoRon significantly inhibited HCT116 CRC cell proliferation and migration in a dose-dependent manner.
  • AdipoRon treatment led to increased apoptosis and G1 phase cell cycle arrest.
  • AdipoRon induced AMPKα phosphorylation and decreased STAT3 expression.

Conclusions:

  • AdipoRon demonstrates significant anti-cancer effects by inhibiting CRC cell proliferation and migration.
  • These effects are mediated through the upregulation of pAMPKα and downregulation of STAT3.
  • AdipoRon shows promise as a potential therapeutic agent for colorectal cancer.
Keywords:
AMPKAdipoRonAdiponectinSTAT3colorectal cancer

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