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Multiplex Cardiac Biomarker Profiling for Precision Coronary Artery Disease Risk Assessment Using SERS with Cubic
Tuotuo Zhang1, Bin Li1,2, Alain Wuethrich3
1College of Chemistry, Central China Normal University, Wuhan 430079, China.
Insights
A novel surface-enhanced Raman scattering (SERS) platform precisely detects coronary artery disease (CAD) by profiling six cardiac biomarkers in serum. This technology aids early risk assessment for conditions like myocardial infarction (MI).
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Cardiovascular Diagnostics
Background:
- Early risk assessment of coronary artery disease (CAD) is vital for preventing severe outcomes like myocardial infarction (MI).
- Current diagnostic methods may lack the sensitivity or specificity for early and precise CAD evaluation.
- Circulating biomarkers hold potential for non-invasive CAD detection.
Purpose of the Study:
- To develop and validate a surface-enhanced Raman scattering (SERS) platform for simultaneous profiling of six cardiac biomarkers in human serum.
- To establish a reliable 'cardiac signature' for accurate CAD risk assessment.
- To enhance the sensitivity and specificity of biomarker detection for clinical applications.
Main Methods:
- Integration of a gold microelectrode and cubic core-satellite nanostructures for highly sensitive SERS detection.
- Utilizing Raman reporters to create signal-amplifying 'hot spots' within nanogaps.
- Employing electrohydrodynamics (EHD) to minimize nonspecific binding and improve detection specificity.
Main Results:
- Achieved a low limit of detection (LOD) of 7.4 fg/mL for lactate dehydrogenase B (LDHB).
- The developed SERS platform successfully generated a distinct cardiac signature.
- This signature effectively differentiated CAD and MI from other cardiac-related disorders (CRDs) in clinical samples.
Conclusions:
- The SERS platform offers a sensitive and specific method for simultaneous detection of multiple cardiac biomarkers.
- The established 'cardiac signature' shows significant potential for early and accurate CAD and MI diagnosis.
- This technology paves the way for improved clinical management of cardiovascular diseases.
Abstract:
An early and accurate risk assessment of coronary artery disease (CAD) is crucial for implementing effective health management strategies before the disease progresses to severe or life-threatening conditions such as myocardial infarction (MI). To this end, we present a surface-enhanced Raman scattering (SERS) platform designed to simultaneously profile six circulating biomarkers in human serum, establishing a cardiac signature for precise CAD evaluation. This SERS platform integrates a gold microelectrode and a cubic core-satellite nanostructure as key components to achieve highly sensitive target detection with a limit of detection as low as 7.4 fg/mL for LDHB. By using Raman reporters as the natural bridges between the core and satellites, numerous "hot spots" were created within the nanogaps that significantly amplify the SERS signal output. Additionally, this SERS platform utilizes electrohydrodynamics (EHD) to selectively remove nonspecific binding events, further enhancing cardiac biomarker detection specificity. Notably, the acquired cardiac signature effectively distinguishes CAD and MI from other cardiac-related disorders (CRDs) in clinical samples, highlighting its potential for clinical applications.
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