An isocratic reverse-phase high-performance liquid chromatographic assay for adenine nucleotides in rat liver

An isocratic, reverse-phase high-performance liquid chromatographic assay for adenine nucleotides was developed and validated for rat liver. The hepatic concentration of adenine nucleotides was shown to be dependent on the method used to obtain the liver sample. The recovery of nucleotides was greater than 90%. The method has the advantages of being specific, fast, and low cost.