Related Experiment Video
Updated: Sep 17, 2025
Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR
Published on: February 14, 2016
RPA Workflow for Sex Determination Genotyping in a Dioecious Plant
Anthony Torres1, Reginald Gaudino2,1
1Cannabis Research Institute, Discovery Partners Institute, University of Illinois System, Chicago, IL, USA.
None:
Field-based techniques for genotyping and identification are facilitated by isothermal amplification and detection technology. This chapter focuses on developing a rapid and field-deployable method for early sex identification genotyping in plants. Leveraging the Y-chromosome-specific regions or gene segments, we designed and evaluated an alternative isothermal amplification assay through recombinase polymerase amplification (RPA) to detect male-specific DNA sequences and perform sex determination genotyping.
More Related Videos
12:10An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
Published on: November 30, 2014
07:12Determination of Self-Incompatibility and Inter-Incompatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses
Published on: June 30, 2023
Related Concept Videos
02:45The Ratio of X Chromosome to Autosomes
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
02:27Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...