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High-Throughput Genotyping by CD-PCR
Yoichi Nakanishi1, Shunta Taga2
1Laboratory of Biochemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan. nakanish@agr.nagoya-u.ac.jp.
Methods in Molecular Biology (Clifton, N.J.)
|June 28, 2025
Abstract:
The process of extracting genomic DNA from biological samples is a bottleneck in PCR genotyping. Here, we describe a speedy genotyping method that combines simple genomic DNA extraction using hot alkaline/SDS and direct use of the extract in an SDS-tolerant cyclodextrin-PCR (CD-PCR).
