Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

Overview
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Structure of the Chemotype Determining Locus in <i>Cannabis sativa</i>.

Plant direct·2026
Same author

Transcriptomic analysis of fatty acid and cannabinoid synthases in an S1 cannabis progeny segregating for propyl cannabinoid biosynthesis.

Plant molecular biology·2026
Same author

Loop-Mediated Isothermal Amplification (LAMP) Genotyping for Presence-Absence Determination of Gene of Interest in Test Samples.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

RPA Workflow for Sex Determination Genotyping in a Dioecious Plant.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Probe-Based RT-qPCR Workflow for Dual Interrogation of Virus/Viroid Genotype in Molecular Diagnostics Multiplex.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Haplotype and Presence-Absence Variation Analysis Using High-Resolution Melting (HRM) Analysis for Genotyping Genes of Interest.

Methods in molecular biology (Clifton, N.J.)·2025

Related Experiment Video

Updated: Jun 1, 2026

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
12:01

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

Published on: February 20, 2012

31.8K

Probe-Based qPCR Workflow Using Fast Nucleic Acid Sampling and Molecular Genotyping in a Dioecious Plant.

Anthony Torres1, Reginald Gaudino1,2

  • 1Cannabis Research Institute, Discovery Partners Institute, University of Illinois System, Chicago, IL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 28, 2025
PubMed
Summary

This study details a probe-based quantitative polymerase chain reaction (qPCR) method for plant genotyping. The protocol efficiently identifies male-specific genotypes from various DNA sources, including crude extracts.

Keywords:
AmpliconCycle thresholdDNADetectionFTA cardsGenotypingMale-specific genotypePCRPlant samplesPreamplificationPreamplification enrichmentPrimersProbeProbe-based detectionqPCRqPCR

More Related Videos

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity
12:42

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Published on: April 13, 2012

12.4K
Genotyping of Plant and Animal Samples without Prior DNA Purification
11:00

Genotyping of Plant and Animal Samples without Prior DNA Purification

Published on: September 24, 2012

31.3K

Related Experiment Videos

Last Updated: Jun 1, 2026

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
12:01

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

Published on: February 20, 2012

31.8K
Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity
12:42

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Published on: April 13, 2012

12.4K
Genotyping of Plant and Animal Samples without Prior DNA Purification
11:00

Genotyping of Plant and Animal Samples without Prior DNA Purification

Published on: September 24, 2012

31.3K

Area of Science:

  • Plant genetics
  • Molecular biology
  • Biotechnology

Background:

  • Accurate plant genotyping is crucial for breeding and research.
  • Traditional methods can be labor-intensive and require high-purity DNA.
  • Need for high-throughput, specific genotyping adaptable to various sample types.

Purpose of the Study:

  • To present a robust protocol for probe-based quantitative polymerase chain reaction (qPCR) genotyping.
  • To enable male-specific genotype identification in plant samples.
  • To demonstrate the method's efficacy with various sample types, including crude extracts.

Main Methods:

  • DNA isolation from raw tissue or FTA cards.
  • Preamplification enrichment reaction of isolated DNA.
  • Probe-based quantitative polymerase chain reaction (qPCR) for genotype detection.

Main Results:

  • Successful male-specific genotype identification in plant DNA.
  • High specificity achieved for genotyping various sample types.
  • Method is robust even with crudely prepared nucleic acid extracts.

Conclusions:

  • The described probe-based qPCR protocol is a high-throughput and specific method for plant genotyping.
  • It is effective for male-specific genotype identification using diverse DNA sources.
  • The protocol's adaptability to crude extracts enhances its practical application in plant science.