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Microbial Biosensors01:17

Microbial Biosensors

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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
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Bacterial Detection & Identification Using Electrochemical Sensors
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Turn-On-Fluorescence Sensor and Solid Sensory Chip for Cyanide Detection.

Muhammad Zahid1, Nadeem Raza2, Samuel Bonne3

  • 1College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, 030006, PR China.

Journal of Fluorescence
|June 28, 2025
PubMed
Summary
This summary is machine-generated.

A novel triazole-ferric complex acts as a selective sensor for cyanide ions, turning non-emissive to fluorescent upon detection. This chemodosimetric probe offers a sensitive method for environmental monitoring of toxic cyanide.

Keywords:
ChemodosimeterChromogenicCyanide ionFluorescenceSensorSensory chipTurn-on

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Area of Science:

  • Coordination Chemistry
  • Analytical Chemistry
  • Materials Science

Background:

  • Development of selective and sensitive probes for anionic species is crucial for environmental monitoring.
  • Cyanide (CN⁻) is a highly toxic anion requiring efficient detection methods.
  • Triazole-ferric complexes offer potential as photophysical sensors due to their tunable properties.

Purpose of the Study:

  • To synthesize and characterize a triazole-ferric complex as a chemodosimetric probe.
  • To evaluate the photophysical response of the probe towards various anionic species.
  • To establish the selectivity and sensitivity of the probe for cyanide detection.

Main Methods:

  • Synthesis of a triazole-ferric complex.
  • Photophysical characterization (absorption and fluorescence spectroscopy) upon treatment with various anions.
  • Fluorescence and emission titration to determine stoichiometry and limit of detection (LOD).
  • Fabrication of the probe on porous silicon for visual detection.

Main Results:

  • The triazole-ferric complex selectively detected cyanide ions (CN⁻) among tested anions, exhibiting a distinguishable absorption pattern.
  • Upon interaction with KCN, the non-emissive probe generated significant fluorescence at 478 nm (excited at 356 nm) with an intensity of 1207 a.u.
  • Fluorescence titration confirmed an equimolar stoichiometric relationship and a low LOD of 0.321 × 10⁻⁷ M for cyanide.
  • The probe immobilized on porous silicon showed a visible color change from blackish-yellow to yellow upon exposure to cyanide.

Conclusions:

  • The synthesized triazole-ferric complex functions as an effective chemodosimetric sensor for selective and sensitive detection of cyanide.
  • The probe's mechanism involves the displacement of a quenched fluorescent moiety by cyanide ions, leading to fluorescence enhancement.
  • The probe demonstrates practical applicability for visual and instrumental detection of cyanide in environmental samples.