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Updated: Aug 17, 2026

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
Published on: March 31, 2011
Protocol for differentiation of human embryonic stem cells into surface epithelium and functional keratinocytes
Jiafeng Liu1, Huaxing Huang1, Hong Ouyang2
1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou 510060, China.
Abstract:
The normal development and function of the epidermis and other ectodermal appendages depend on the proper formation of the surface epithelium (SE). Here, we present a protocol to differentiate human embryonic stem cells (hESCs) into SE and subsequently into epidermal keratinocyte progenitors. We then detail procedures for the passage and expansion of keratinocyte progenitors, followed by 2D validation of terminal differentiation and 3D assessment of stratification potential. This protocol represents a promising advancement for stem cell-based regenerative medicine. For complete details on the use and execution of this protocol, please refer to Liu et al.1 and Huang et al.2.
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